納米金和釓摻雜氧化鋅量子點(diǎn)納米載體改善化療效果
發(fā)布時間：2021-08-13 17:22
在過去二十年里,納米技術(shù)因其覆蓋了多學(xué)科領(lǐng)域并且具有改變世界的巨大潛力,對人類的生活產(chǎn)生了巨大影響。納米技術(shù)是當(dāng)今世界研究熱點(diǎn)之一,在人類日常生活的各個領(lǐng)域都有廣泛的應(yīng)用。大量納米結(jié)構(gòu)的合成推動了多科學(xué)領(lǐng)域的進(jìn)一步深化研究,這些研究領(lǐng)域包括但不限于半導(dǎo)體器件、傳感、太陽能電池、生物醫(yī)學(xué)和納米藥物。盡管已經(jīng)合成了許多在電子器件制造方面有潛在應(yīng)用前景的納米結(jié)構(gòu),但是在健康醫(yī)療方面,納米顆粒（NPs）仍然面臨大量的挑戰(zhàn),例如高毒性等。此外,納米顆粒（NPs）在細(xì)胞或組織內(nèi)的積聚也是一個有待解決的問題。為此人們開展了復(fù)合的納米顆粒研究,復(fù)合納米顆粒可以同時執(zhí)行多個任務(wù),在化學(xué)分析、催化、儲能和藥物等領(lǐng)域具有廣闊的前景。例如,在醫(yī)學(xué)中,復(fù)合納米顆�？梢酝瑫r作為藥物載體和診斷探針。在傳統(tǒng)的藥物輸送系統(tǒng)中,采用口服、靜脈注射或通過皮膚吸收等模式給藥。這些方法都具有血藥濃度高、載藥/釋藥能力差、靶點(diǎn)選擇性差等缺點(diǎn),具有長效副作用,危害人類健康。另一方面,慢性疾病如癌癥嚴(yán)重威脅著生命,但其治療在發(fā)展中國家和發(fā)達(dá)國家都仍然存在很多問題,例如腫瘤和癌癥治療專家在癌癥診斷和治療時會遇到多藥耐藥（MDR）限制化...
【文章來源】：電子科技大學(xué)四川省 211工程院校 985工程院校 教育部直屬院校
【文章頁數(shù)】：119 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
abstract
Chapter 1 Introduction
1.1 General Introduction
1.2 Nanomaterial Systems (NMs)
1.2.1 Core-shell Nanoparticle Systems
1.2.2 Multifunctional Mesoporous Silica (MMS) Nanoparticles
1.2.3 Magnetic Nanoparticles (MNPs)
1.2.4 Quantum Dots
1.2.5 Gold Nanoparticles
1.3 Properties of Nanoparticle
1.3.1 Core Material
1.3.2 Shape and Size
1.3.3 Surface Chemistry
1.4 Objectives
1.5 Thesis Outlines
Chapter 2 Biomedical Applications of Hybrid Nanomaterial
2.1 Introduction
2.2 Cancer: an Overview
2.2.1 Symptoms, Causes, Epidemiology and Cost
2.2.2 Cancer Classification and How it Kills
2.3 Cancer Diagnostics and Treatments in Use Today
2.3.1 Cancer Treatments in Use Today
2.4 Hybrid Nanomaterials Applications in Cancer Treatment
2.5 Uptake and Toxicity of Hybrid Nanomaterial
Chapter 3 Materials, Methods and Characterization Techniques
3.1 Introduction
3.2 Materials
3.2.1 Reagents for Gold Nanoparticles
3.2.2 Reagents for Gadolinium-doped Zinc Oxide Quantum Dots
3.2.3 Surfactant and Chemotherapeutic Agents
3.2.4 Cell Line and Culturing Reagents
3.2.5 Instruments for Analysis
3.3 Method
3.3.1 Synthesis of Gold Nanoparticles
3.3.2 DOX Loading on S1
3.3.3 Nanodrug Formulation; BLM Loading onto S1 and S2 Surfaces
3.3.4 Synthesis of Gadolinium-doped Zinc Oxide (Gd@ZnO) QDs
3.3.5 Functionalization of Gd@ZnO QDs
3.3.6 PEGylation of APTES- Gd@ZnO
3.3.7 Preparation of Nanodrug; DOX Loading
3.4 Functionalization Techniques
3.4.1 Centrifugation Technique
3.4.2 Ultra-sonication
3.4.3 Drug Loading and Release Experiment
3.4.4 Cell Culturing Experiment
3.5 Cell Viability and Cell Imaging
3.6 Statistical Analysis
3.7 Material Characterization
3.7.1 X-ray Diffractometer (XRD)
3.7.2 Dynamic Light Scattering (DLS)
3.7.3 Nanoparticle Tracking Analysis (NTA)
3.7.4 Zeta Potential Measurements
3.7.5 Ultraviolet–visible (UV-Vis) Spectroscopy
3.7.6 Fourier Transform Infrared (FTIR) Spectroscopy
3.7.7 Transmission Electronic Microscopy (TEM)
3.7.8 Laser Scanning Confocal Microscopy (LSCM)
3.8 Conclusion
Chapter 4 Characterization and Functionalization of Hybrid Nanomaterial
4.1 Introduction
4.1.1 Characterization of the Naked and Functionalized Gold Nanoparticles .
4.1.2 Characterization of the Naked and Functionalized Gd@ZnO QDs
4.2 Conclusion
Chapter 5 Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to He La Cells
5.1 Introduction
5.2 Material Characterization
5.3 Flow Cytometric Analysis and LC50, EC50 Determination
5.4 Cellular Internalization and Imaging of He La Cells
5.5 Results and Discussion
5.5.1 Nano-drug Formulations
5.5.2 Nano-drug Characterizations
5.5.2.1 XRD, UV-vis and FTIR Analysis
5.5.2.2 Drug Conjugation Analysis
5.5.2.3 TEM and HRTEM Analysis
5.5.2.4 Nanoparticles Tracking Analysis
5.5.2.5 Cytotoxicity and LC50, EC50 Study
5.5.2.6 Cell Cycle Determination
5.5.2.7 Cellular Internalization and LSCM Imaging
5.5.2.8 NIR Triggered Release Experiment
5.5.2.9 Comparative Analysis
5.6 Conclusion
Chapter 6 Synthesis of Gd@ZnO Quantum Dots and Design of p H-responsive Unique Drug Delivery System
6.1 Introduction
6.2 Results and Discussion
6.2.1 Synthesis of Gd@ZnO QDs
6.2.2 Characterization of Gd@ZnO QDs
6.2.2.1 X-ray diffraction and TEM Analysis
6.2.2.2 DLS Measurements
6.2.2.3 UV-vis and FTIR Analysis
6.2.2.4 Drug Loading/Release Experiment
6.3 Conclusions
Chapter 7 Conclusion and Future Work
7.1 Conclusion
7.2 Advantages and Disadvantages
7.3 Perspective
7.4 Future Work
Acknowledgement
References
Research Results Obtained During the Study for Doctoral Degree
本文編號：3340837
【文章來源】：電子科技大學(xué)四川省 211工程院校 985工程院校 教育部直屬院校
【文章頁數(shù)】：119 頁
【學(xué)位級別】：博士
【文章目錄】：
摘要
abstract
Chapter 1 Introduction
1.1 General Introduction
1.2 Nanomaterial Systems (NMs)
1.2.1 Core-shell Nanoparticle Systems
1.2.2 Multifunctional Mesoporous Silica (MMS) Nanoparticles
1.2.3 Magnetic Nanoparticles (MNPs)
1.2.4 Quantum Dots
1.2.5 Gold Nanoparticles
1.3 Properties of Nanoparticle
1.3.1 Core Material
1.3.2 Shape and Size
1.3.3 Surface Chemistry
1.4 Objectives
1.5 Thesis Outlines
Chapter 2 Biomedical Applications of Hybrid Nanomaterial
2.1 Introduction
2.2 Cancer: an Overview
2.2.1 Symptoms, Causes, Epidemiology and Cost
2.2.2 Cancer Classification and How it Kills
2.3 Cancer Diagnostics and Treatments in Use Today
2.3.1 Cancer Treatments in Use Today
2.4 Hybrid Nanomaterials Applications in Cancer Treatment
2.5 Uptake and Toxicity of Hybrid Nanomaterial
Chapter 3 Materials, Methods and Characterization Techniques
3.1 Introduction
3.2 Materials
3.2.1 Reagents for Gold Nanoparticles
3.2.2 Reagents for Gadolinium-doped Zinc Oxide Quantum Dots
3.2.3 Surfactant and Chemotherapeutic Agents
3.2.4 Cell Line and Culturing Reagents
3.2.5 Instruments for Analysis
3.3 Method
3.3.1 Synthesis of Gold Nanoparticles
3.3.2 DOX Loading on S1
3.3.3 Nanodrug Formulation; BLM Loading onto S1 and S2 Surfaces
3.3.4 Synthesis of Gadolinium-doped Zinc Oxide (Gd@ZnO) QDs
3.3.5 Functionalization of Gd@ZnO QDs
3.3.6 PEGylation of APTES- Gd@ZnO
3.3.7 Preparation of Nanodrug; DOX Loading
3.4 Functionalization Techniques
3.4.1 Centrifugation Technique
3.4.2 Ultra-sonication
3.4.3 Drug Loading and Release Experiment
3.4.4 Cell Culturing Experiment
3.5 Cell Viability and Cell Imaging
3.6 Statistical Analysis
3.7 Material Characterization
3.7.1 X-ray Diffractometer (XRD)
3.7.2 Dynamic Light Scattering (DLS)
3.7.3 Nanoparticle Tracking Analysis (NTA)
3.7.4 Zeta Potential Measurements
3.7.5 Ultraviolet–visible (UV-Vis) Spectroscopy
3.7.6 Fourier Transform Infrared (FTIR) Spectroscopy
3.7.7 Transmission Electronic Microscopy (TEM)
3.7.8 Laser Scanning Confocal Microscopy (LSCM)
3.8 Conclusion
Chapter 4 Characterization and Functionalization of Hybrid Nanomaterial
4.1 Introduction
4.1.1 Characterization of the Naked and Functionalized Gold Nanoparticles .
4.1.2 Characterization of the Naked and Functionalized Gd@ZnO QDs
4.2 Conclusion
Chapter 5 Gold Nanoparticles-enabled Efficient Dual Delivery of Anticancer Therapeutics to He La Cells
5.1 Introduction
5.2 Material Characterization
5.3 Flow Cytometric Analysis and LC50, EC50 Determination
5.4 Cellular Internalization and Imaging of He La Cells
5.5 Results and Discussion
5.5.1 Nano-drug Formulations
5.5.2 Nano-drug Characterizations
5.5.2.1 XRD, UV-vis and FTIR Analysis
5.5.2.2 Drug Conjugation Analysis
5.5.2.3 TEM and HRTEM Analysis
5.5.2.4 Nanoparticles Tracking Analysis
5.5.2.5 Cytotoxicity and LC50, EC50 Study
5.5.2.6 Cell Cycle Determination
5.5.2.7 Cellular Internalization and LSCM Imaging
5.5.2.8 NIR Triggered Release Experiment
5.5.2.9 Comparative Analysis
5.6 Conclusion
Chapter 6 Synthesis of Gd@ZnO Quantum Dots and Design of p H-responsive Unique Drug Delivery System
6.1 Introduction
6.2 Results and Discussion
6.2.1 Synthesis of Gd@ZnO QDs
6.2.2 Characterization of Gd@ZnO QDs
6.2.2.1 X-ray diffraction and TEM Analysis
6.2.2.2 DLS Measurements
6.2.2.3 UV-vis and FTIR Analysis
6.2.2.4 Drug Loading/Release Experiment
6.3 Conclusions
Chapter 7 Conclusion and Future Work
7.1 Conclusion
7.2 Advantages and Disadvantages
7.3 Perspective
7.4 Future Work
Acknowledgement
References
Research Results Obtained During the Study for Doctoral Degree
本文編號：3340837
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