發(fā)布時(shí)間：2019-06-20 04:42

【摘要】：目的:建立高效液相色譜法檢測(cè)干血濾紙片中卡馬西平血藥濃度的方法。方法:將30μL含卡馬西平的全血滴在濾紙上,室溫下晾干至少3 h,待干透后從濾紙上打孔取下6 mm直徑的血斑,放入1.5 mL普通離心管中,加入含有內(nèi)標(biāo)的甲醇溶液200μL,超聲水浴10 min,渦旋1 min,15 000 r·min~(-1)離心5 min,取上清進(jìn)樣。色譜柱為AgilentTC-C_(18)柱(250 mm×4.6 mm,5μm),流動(dòng)相為甲醇-水(55∶45),內(nèi)標(biāo)為苯妥英,流速為1.0mL·min~(-1),檢測(cè)波長(zhǎng)為235 nm。結(jié)果:卡馬西平血藥濃度在1.0~32.0μg·mL~(-1)范圍內(nèi)與峰面積呈良好線性關(guān)系(r=0.999 0),方法回收率為101.3%~110.5%,提取回收率為59.8%~62.1%。結(jié)論:本方法操作簡(jiǎn)單、準(zhǔn)確、可靠,適用于測(cè)定干血濾紙片中卡馬西平的血藥濃度。
[Abstract]:Objective: to establish a HPLC method for the determination of carbamazepine in dry blood filter paper. Methods: 30 渭 L whole blood droplets containing carbamazepine were dripped on the filter paper and dried at room temperature for at least 3 H. after drying through, the blood spots of 6 mm diameter were removed from the filter paper and put into 1.5 mL centrifuge tube. 200 渭 L methanol solution containing internal standard was added, and 1 min,15 000 r 路min ~ (- 1) centrifugation 5 min, with 10 min, vortex in ultrasonic water bath was used to take the sample. The chromatographic column was AgilentTC-C_ (18) column (250 mm 脳 4.6 mm, 5 渭 m), the mobile phase was methanol-water (55:45), the internal standard was phenytoin, the flow rate was 1.0mL 路min ~ (- 1), and the detection wavelength was 235 nm.. Results: there was a good linear relationship between carbamazepine and peak area in the range of 1.0 ~ 32.0 渭 g 路mL ~ (- 1) (r 鈮，

本文編號(hào)：2502940