【摘要】：目的:建立同時測定復(fù)方地克軟膏中氯霉素、克霉唑和醋酸地塞米松含量的方法。方法:采用高效液相色譜法。色譜柱為Thermo Hypersil BDS C18,流動相為甲醇-0.05 mol/L磷酸二氫鉀(p H=5.7)(梯度洗脫),流速為1.0 ml/min,檢測波長為235 nm,柱溫為35℃,進樣量為10μl。結(jié)果:氯霉素、克霉唑、醋酸地塞米松檢測質(zhì)量濃度分別在0.10~0.40、0.05~0.20、0.03~0.12 mg/ml范圍內(nèi)與峰面積呈良好的線性關(guān)系;精密度、穩(wěn)定性、重復(fù)性試驗的RSD2%;回收率分別為95.42%~103.28%、97.12%~102.96%、95.10%~102.69%,RSD分別為2.8%、1.8%、2.9%(n=9)。結(jié)論:該方法簡便快速、結(jié)果準確、重復(fù)性好,適用于復(fù)方地克軟膏及相關(guān)制劑的質(zhì)量控制。
[Abstract]:Objective: to establish a method for the simultaneous determination of chloramphenicol, clotrimazole and dexamethasone acetate in compound Dike ointment. Methods: high performance liquid chromatography (HPLC) was used. The chromatographic column was Thermo Hypersil BDS C 18, the mobile phase was methanol-0.05 mol/L potassium dihydrogen phosphate (pH 鈮，

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