【摘要】：第一部分阿托伐他汀對人臍靜脈內(nèi)皮細(xì)胞Adropin相關(guān)基因及蛋白表達(dá)的影響 目的 探討阿托伐他汀對人臍靜脈內(nèi)皮細(xì)胞（human umbilical endothelial cell, HUVEC）Adropin相關(guān)基因及蛋白表達(dá)的影響。 方法 將不同濃度的阿托伐他�。�0.002umol/l、0.02umol/l、0.2umol/l、2umol/l、20umol/l）與HUVEC共培養(yǎng)6小時（hour，h）、12h、24h。采用MTT法檢測HUVEC的增殖情況。半定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)法檢測HUVEC Adropin mRNA的表達(dá)。ELISA法測定細(xì)胞上清液中Adropin蛋白的表達(dá)。 結(jié)果 1.不同濃度的阿托伐他汀與HUVEC共培養(yǎng)6h時，20umol/l濃度組增殖活力明顯高于對照組（P0.05），其他各濃度組差異無統(tǒng)計(jì)學(xué)意義。共培養(yǎng)12h時，0.02umol/l、0.2umol/l、2umol/l、20umol/l濃度組細(xì)胞活力顯著高于對照組（P0.05），0.2umol/l、2umol/l、20umol/l濃度組活力顯著高于0.002umol/l濃度組（P0.05）。共培養(yǎng)24h時，各濃度組細(xì)胞活力均顯著高于對照組（P0.05），，0.02umol/l、0.2umol/l、2umol/l、20umol/l濃度組活力顯著高于0.002umol/l濃度組(P0.05)。 同一濃度阿托伐他汀分別與HUVEC共培養(yǎng)6h、12h、24h，12h和24h組活力明顯高于6h組（P0.05）。 2.不同濃度組阿托伐他汀與HUVEC共培養(yǎng)6h、12h、24h后，Adropin mRNA的表達(dá)均顯著高于對照組（P0.001），0.02umol/l、0.2umol/l、2umol/l、20umol/l濃度組Adropin mRNA的表達(dá)顯著高于0.002umol/l組（P0.001）。 同一濃度阿托伐他汀分別與HUVEC共培養(yǎng)6h、12h、24h，12h和24h組mRNA表達(dá)量明顯高于6h組（P0.01）。 3.不同濃度的阿托伐他汀與HUVEC分別共培養(yǎng)6h、12h、24h，各濃度組HUVEC上清中Adropin蛋白的表達(dá)顯著高于對照組（P0.001），0.02umol/l、0.2umol/l、2umol/l、20umol/l濃度組Adropin蛋白的表達(dá)顯著高于0.002umol/l組（P0.001）。 同一濃度阿托伐他汀分別與HUVEC共培養(yǎng)6h、12h、24h時，12h和24h組蛋白表達(dá)量明顯高于6h組(P0.001）。 4. Adropin蛋白含量與HUVEC吸光度值（OD值）進(jìn)行Pearson相關(guān)性分析顯示不同時間段各濃度組adropin與OD值呈顯著正相關(guān)（6h，相關(guān)系數(shù)r=0.471，P=0.049；12h，r=0.756，P=0.001；24h，r=0.725，P=0.001)。 結(jié)論 1.阿托伐他汀能夠促進(jìn)HUVEC的增殖，并呈劑量和時間依賴性。 2.阿托伐他汀能夠促進(jìn)HUVEC Adropin相關(guān)基因及蛋白的表達(dá)，且呈劑量和時間依賴性。 3. HUVEC的增殖活力與其Adropin蛋白的表達(dá)呈正相關(guān)。 第二部分阿托伐他汀對大鼠胸主動脈平滑肌細(xì)胞Adropin相關(guān)基因及蛋白表達(dá)的影響 目的 探討阿托伐他汀對大鼠胸主動脈平滑肌細(xì)胞（rat aortic smooth muscle cell，RASMC）Adropin相關(guān)基因及蛋白表達(dá)的影響。 方法 原代培養(yǎng)SD大鼠的RASMC，免疫熒光和免疫細(xì)胞化學(xué)染色法進(jìn)行鑒定，取4-5代細(xì)胞進(jìn)行試驗(yàn)。.不同濃度的阿托伐他�。�0.02umol/l、0.2umol/l、2umol/l、20umol/l）與RASMC分別共培養(yǎng)6h、12h、24h。采用MTT法檢測RASMC的增殖情況。半定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)法檢測RASMCAdropin mRNA的表達(dá)。ELISA法測定細(xì)胞上清液中Adropin蛋白的表達(dá)。 結(jié)果 1.不同濃度的阿托伐他汀與RASMC共培養(yǎng)24h時，各濃度組細(xì)胞活力顯著低于對照組（P0.05），0.02umol/l、0.2umol/l阿托伐他汀組活力明顯高于20umol/組（P0.05）。 同一濃度阿托伐他汀與RASMC共培養(yǎng)6h、12h、24h，12h和24h組活力明顯低于6h組（P0.05）。 2.不同濃度的阿托伐他汀與RASMC共培養(yǎng)，各濃度組Adropin mRNA表達(dá)量均高于對照組（P0.05）；隨著濃度增大，細(xì)胞中Adropin mRNA表達(dá)量逐漸降低（P0.05）。 同一濃度阿托伐他汀分別與RASMC共培養(yǎng)6h、12h、24h，12h和24h組AdropinmRNA表達(dá)量明顯高于6h組（P0.01）。 3.不同濃度的阿托伐他汀與RASMC分別共培養(yǎng)6h、12h、24h，各濃度組RASMC上清中Adropin蛋白的表達(dá)顯著高于對照組（P0.05）。隨著濃度增大，細(xì)胞中Adropin蛋白的表達(dá)量逐漸降低（P0.05）。 同一濃度的阿托伐他汀分別與RASMC共培養(yǎng)6h、12h、24h，Adropin蛋白的表達(dá)量隨著時間延長明顯增加（P0.05）。 4. Adropin蛋白含量與RASMC吸光度值（OD值）進(jìn)行Pearson相關(guān)性分析顯示不同時間段各濃度組adropin與OD值呈顯著正相關(guān)(6h，相關(guān)系數(shù)r=0.694，P=0.012)vs(12h，r=0.697，P=0.002) vs(24h，r=0.826，P=0.001)。 結(jié)論 1.阿托伐他汀能抑制RASMC的增殖并呈劑量和時間依賴性。 2.阿托伐他汀能促進(jìn)RASMC Adropin相關(guān)基因及蛋白的表達(dá)，但隨濃度的增加其促進(jìn)作用逐漸減弱。 3. RASMC的增長活力與其Adropin蛋白的表達(dá)呈正相關(guān)。
[Abstract]:The effect of the first part of atorvastatin on the expression of the related gene and protein of human umbilical vein endothelial cell Objective To study the expression of Atorvastatin on human umbilical vein endothelial cells (HUVEC) and its expression in human umbilical vein endothelial cells (HUVEC). a shadow in response to that method, different concentrations of atorvastatin (0.002 mol/ l, 0.02 mol/ l, 0.2 mol/ l,2 umol/ l,20 umol/ l) were co-cultured with HUVEC for 6 h (hour, h), 12 h,24 h. HUV was detected by MTT method. The proliferation of EC was detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Expression of mRNA. Adrotp in the supernatant of the cells by ELISA in Results 1. The proliferation of Atorvastatin and HUVEC at different concentrations was significantly higher than that in the control group (P0.05). There was no statistical significance in the concentration group. In the co-culture for 12 h, the activity of the cell in the concentration group was significantly higher than that of the control group (P0.05), the cell viability of the concentration group was significantly higher than that of the control group (P0.05), and the activity of the concentration group of 0.2 umol/ l,2 umol/ l and 20 umol/ l was significantly higher than that of the control group (P0.05). The activity of cells in each concentration group was significantly higher than that of the control group (P0.05), 0.02 mol/ l, 0.2 uml/ l,2 umol/ l and 20 umol/ l in the concentration group (P0.05), and the activity of the concentration group was significantly higher than that of the control group (P0.05). The same concentration of atorvastatin were co-cultured with HUVEC for 6 h,12 h,24 h,12 h and 24 h, respectively. After 6 h,12 h and 24 h of co-culture of Atorvastatin and HUVEC in different concentration groups, the expression of Adropin mRNA was significantly higher than that in the control group (P 0.001), 0.02 mol/ l, 0.2 umol/ l,2 umol/ l and 20 umol/ l. The same concentration of atorvastatin were co-cultured with HUVEC for 6 h,12 h,24 h,12 h and 24 h, respectively. Atorvastatin and HUVEC were co-cultured for 6 h,12 h and 24 h, respectively. The expression of Atropin in HUVEC was significantly higher than that of control group (P 0.001), 0.02 mol/ l, 0.2 uml/ l,2 umol/ l and 20 umol/ l. The same concentration of atorvastatin were co-cultured with HUVEC for 6 h,12 h,24 h,12 h and 24 h, respectively. The expression of histone was significantly higher than that of the 6 h group (P0.001).4. The Pearson correlation analysis showed that the concentration of adropin was positively correlated with the OD (r = 0.471, P = 0.049; 12h, r = 0.756, P = 0.0) for different time periods. 01 ;24 h, r = 0.725, P = 0.001). Conclusion 1. torvastatin can promote the proliferation of HUVEC and dose and time-dependent.2. Atorvastatin can promote HUVE The expression of C-Adropin-related gene and protein is dose-dependent and time-dependent. 3. The proliferation activity of HUVEC is positively related to the expression of its Adropin protein. cut him Objective To study the effect of statins on the expression of the related gene and protein of the rat thoracic aortic smooth muscle cells, and to investigate the effect of atorvastatin on the rat thoracic aortic smooth muscle cells h m The effect of the expression of the related gene and protein of the rat cell (RASMC) D-rat RASMC, immunofluorescence and immunocytochemical staining for identification,4-5 generation of cells tested.. different concentrations of atorvastatin (0.02 uml/ l, 0.2 umol /l,2umol/l,20umol/l And were co-cultured with RASMC for 6 h,12 h and 24 h, respectively. C. Detection of RASMC by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) Ad Hoc Results 1. The activity of Atorvastatin and RASMC at different concentrations was significantly lower than that in the control group (P0.05). The activity of the Atorvastatin group was significantly higher than that of 20 umol/ l (P0.05). Atorvastatin and RASMC were co-cultured for 6 h,12 h,24 h,12 h and 24 h for 6 h,12 h,24 h,12 h and 24 h, respectively. At the same concentration, Atorvastatin and RASM were compared with those in the control group (P0.05). C co-culture for 6 h,12 h,24 h,12 h and 24 h was significantly higher than that in 6 h (P0.01).3. Atorvastatin and RASMC at different concentrations 6 h,12 h and 24 h were co-cultured for 6 h,12 h and 24 h. The white expression was significantly higher in the control group than in the control group (P0.05). As the concentration increased, the expression of the Adropin in the cells decreased gradually (P0.05). After 6 h,12 h and 24 h of co-culture with RASMC, the expression of ropin protein increased significantly with the increase of time (P0.05).4. The Pearson correlation analysis showed that the concentration of adropin in each concentration group was positively correlated with the OD value for different time periods (6 h, r = 0.6). 94 ,P=0.012)vs(12h,r=0.697,P=0.0 02) vs (24 h, r = 0.826, P = 0.001). Conclusion 1. Atorvastatin can inhibit the proliferation of RASMC 2. Atorvastatin can promote RASMC Ad
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R96

【相似文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 連i吜

本文編號：2495616