【摘要】：目的建立羅紅霉素的離子液體增敏熒光測定法。方法羅紅霉素在λ_(ex)/λ_(em)=310nm/420nm處產(chǎn)生微弱熒光,加入離子液體(Bmim)PF_6后,熒光強度大大增強,對影響羅紅霉素熒光強度的因素:濃度、酸度、時間、溫度等進行了詳細探究。羅紅霉素在2.0×10~(-7)~8.0×10~(-6)mol/L范圍內(nèi)與其熒光強度有良好的線性關(guān)系,相關(guān)系數(shù)R=0.9989,檢出限1.8×10~(-7)mol/L,回收率在92%~104%。結(jié)果利用本法測定羅紅霉素藥品中有效成分的含量,藥物制劑中羅紅霉素的測定值與標示值相吻合。結(jié)論該法可以作為該制劑含量測定的質(zhì)量控制方法。
[Abstract]:Objective to establish a method for the determination of roxithromycin by ionic liquid sensitized fluorimetry. Methods roxithromycin produced weak fluorescence at 位 _ (ex) / 位 _ (em) = 310nm/420nm. The fluorescence intensity of roxithromycin was greatly enhanced with the addition of ionic liquid (Bmim) PF_6. The factors affecting the fluorescence intensity of roxithromycin were concentration, acidity and time. The temperature and so on have been probed into in detail. The linear range of roxithromycin was 2.0 脳 10 ~ (- 7) ~ 8.0 脳 10 ~ (- 6) mol/L. The correlation coefficient was 0.9989. The detection limit was 1.8 脳 10 ~ (- 7) mol/L,. The recovery of roxithromycin was 92% ~ 10 ~ (- 7) mol/L,. Results the content of roxithromycin in roxithromycin drug was determined by this method. The determination value of roxithromycin in drug preparation was consistent with the marked value. Conclusion this method can be used as a quality control method for the determination of this preparation.
【作者單位】： 忻州師范學院化學系;
【基金】：山西省大學生創(chuàng)新創(chuàng)業(yè)訓練項目(No.2015381) 忻州師范學院大學生科技創(chuàng)新項目(2014) 省級重點實驗室基金資助(No.2014936)
【分類號】：R927.1
，

本文編號：2468382