【摘要】：目的評(píng)價(jià)P-糖蛋白及有機(jī)陽離子轉(zhuǎn)運(yùn)體對(duì)左氧氟沙星跨胃黏膜轉(zhuǎn)運(yùn)的影響。方法用大鼠胃黏膜與Ussing chamber技術(shù)建立胃黏膜體外模型,分為空白組及加入P-糖蛋白抑制劑(環(huán)孢素A)或有機(jī)陽離子轉(zhuǎn)運(yùn)體抑制劑(西咪替丁)的抑制劑組并進(jìn)行雙向轉(zhuǎn)運(yùn)實(shí)驗(yàn),用高效液相色譜法對(duì)左氧氟沙星進(jìn)行定量分析,計(jì)算其表觀滲透系數(shù)(Papp)。結(jié)果加入環(huán)孢素A后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)由2.20變?yōu)?.06(P0.05);加入西咪替丁后,50μg·m L-1左氧氟沙星Papp(S-M)/Papp(M-S)與空白組比較,差異無統(tǒng)計(jì)學(xué)意義。結(jié)論 P-糖蛋白參與左氧氟沙星跨胃黏膜轉(zhuǎn)運(yùn)過程,而有機(jī)陽離子轉(zhuǎn)運(yùn)體不參與左氧氟沙星跨胃黏膜轉(zhuǎn)運(yùn)過程。
[Abstract]:Objective to evaluate the effects of P-glycoprotein and organic cation transporter on transgastric transport of levofloxacin. Methods the rat gastric mucosa model in vitro was established by Ussing chamber technique. They were divided into two groups: blank group, inhibitor of P-glycoprotein (cyclosporin A) or cimetidine (cimetidine), and the two-way transport experiment was carried out, and the quantitative analysis of levofloxacin was carried out by high performance liquid chromatography (HPLC). Calculation of apparent permeability coefficient (Papp). Results after addition of cyclosporine A, 50 渭 g 路mL ~ (- 1) of levofloxacin Papp / Papp (Mus) was changed from 2.20 to 1.06 (P0.05). When cimetidine was added, there was no significant difference between 50 渭 g 路mL-1 levofloxacin Papp / Papp (Mus) group and the blank group. Conclusion P-glycoprotein is involved in the transgastric transport of levofloxacin, but organic cation transporters are not involved in the transgastric transport of levofloxacin.
【作者單位】： 安徽醫(yī)科大學(xué)第一附屬醫(yī)院科研處;安徽省消化系病重點(diǎn)實(shí)驗(yàn)室;安徽醫(yī)科大學(xué)第一附屬醫(yī)院消化內(nèi)科;
【基金】：國(guó)家自然科學(xué)基金青年基金資助項(xiàng)目(81100269)
【分類號(hào)】：R965

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