發(fā)布時(shí)間：2019-02-11 17:04

【摘要】：背景:脂肪干細(xì)胞在GMP潔凈車間制備完成后需要做成制劑才能給患者輸注。低溫制劑相比于凍存制劑有很多優(yōu)勢,然而關(guān)于低溫短期保存對干細(xì)胞生物學(xué)特性影響的研究較少。目的:評價(jià)用含5%人血白蛋白的復(fù)方電解質(zhì)低溫保存24 h對脂肪間充質(zhì)干細(xì)胞生物學(xué)特性的影響。方法:取第3-6代人脂肪間充質(zhì)干細(xì)胞,以5×109 L-1的細(xì)胞濃度重懸在含5%人血白蛋白的復(fù)方電解質(zhì)注射液中,將細(xì)胞懸液轉(zhuǎn)移至凍存管中,置于冷鏈運(yùn)輸箱,2-8℃低溫保存24 h。觀察低溫保存前后的細(xì)胞形態(tài)、貼壁能力、細(xì)胞活率、細(xì)胞直徑及細(xì)胞免疫表型變化。結(jié)果與結(jié)論:(1)脂肪間充質(zhì)干細(xì)胞低溫保存24 h后,死細(xì)胞增多,細(xì)胞活率顯著下降,但細(xì)胞活率仍大于80%,活細(xì)胞直徑顯著增大;(2)脂肪間充質(zhì)干細(xì)胞低溫保存24 h后,少量細(xì)胞為圓形,失去貼壁能力,大部分細(xì)胞能貼壁生長,形態(tài)為梭形,與細(xì)胞保存前貼壁形態(tài)相似;(3)脂肪間充質(zhì)干細(xì)胞低溫保存24 h后,HLA-DR、CD34和CD45均為陰性表達(dá),陽性率低于2%;CD29、CD73和CD105均為陽性表達(dá),陽性率高于95%,但保存后的細(xì)胞顯著地由一群分為兩群,部分細(xì)胞體積增大,向右移動(dòng);(4)結(jié)果表明,低溫制劑保存未明顯影響脂肪間充質(zhì)干細(xì)胞的貼壁生長能力、存活率及免疫表型等干性生物學(xué)特性。
[Abstract]:Background: adipose stem cells need to be prepared after preparation in GMP clean workshop to be infused into patients. Cryopreservation has many advantages over cryopreservation, but there are few studies on the effects of cryopreservation on the biological characteristics of stem cells. Aim: to evaluate the effects of compound electrolyte containing 5% human serum albumin (HSA) on the biological characteristics of adipose mesenchymal stem cells (AMSCs) after cryopreservation for 24 h. Methods: human adipose mesenchymal stem cells (HMSCs) of passage 3-6 were resuspended in 5 脳 109 L-1 cell concentration in compound electrolyte injection containing 5% human serum albumin. The suspension was transferred to the cryopreserved tube and placed in a cold chain transport box. Stored at 2-8 鈩，

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