【摘要】：目的:制備吲哚菁綠金納米籠(ICG/Biotin-PEG-AuNC-PCM),研究其體外抗腫瘤活性。方法:使用多元醇還原法制備銀納米立方體(Ag NC)作為模板,通過離子置換反應(yīng)制備金納米籠(AuNC)。對AuNC形貌、粒徑及近紅外(NIR)光熱性質(zhì)進(jìn)行表征。使用有機溶劑揮發(fā)法制備裝載ICG、1-十四醇(PCM)、表面修飾生物素聚乙二醇巰基(Biotin-PEG-SH)的ICG/Biotin-PEG-AuNC-PCM,檢測其粒徑、多分散系數(shù)(PDI)、載藥量,考察其在37、40℃下180 min內(nèi)的體外累積釋放度(Q)。以耐阿霉素人乳腺癌細(xì)胞(MCF-7/ADR細(xì)胞)為研究對象,采用MTT法考察ICG、ICG/PEG-AuNC-PCM、ICG/Biotin-PEG-AuNC-PCM的抗腫瘤活性,計算半數(shù)抑制濃度(IC50)。結(jié)果:AuNC為立方體,粒徑為60 nm左右,具有良好的光熱性質(zhì)。ICG/Biotin-PEG-AuNCPCM的粒徑為(105±2.8)nm,PDI為0.261±0.02(n=3);載藥量為1.34×108g/mol AuNC;37℃下Q180 min約為10%,40℃下Q20 min約為80%。ICG、ICG/PEG-AuNC-PCM、ICG/Biotin-PEG-AuNC-PCM對MCF-7/ADR細(xì)胞的IC50分別為95.2、29.3、16.1μg/m L。結(jié)論:成功制得ICG/Biotin-PEG-AuNC-PCM,其對MCF-7/ADR細(xì)胞的抗腫瘤活性強于ICG。
[Abstract]:Aim: to prepare indocyanine green gold nanoparticles (ICG/Biotin-PEG-AuNC-PCM) and to study its anti-tumor activity in vitro. Methods: silver nanocrystalline (Ag NC) was prepared by polyol reduction method, and gold nanocage (AuNC). Was prepared by ion replacement reaction. AuNC morphology, particle size and near infrared (NIR) photothermal properties were characterized. ICG/Biotin-PEG-AuNC-PCM, loaded with ICG,1- tetradecanol (PCM), surface modified biotin polyethylene glycol mercapto (Biotin-PEG-SH) was prepared by organic solvent volatilization method to detect the particle size and the amount of drug loaded with polydispersity (PDI),. In vitro cumulative release of (Q). At 37 鈩，

本文編號：2413889