【摘要】：目的:研究吡咯喹啉醌(pyrroloquinoline quinone,PQQ)是否通過(guò)抑制氧化應(yīng)激發(fā)揮對(duì)Bmi-1缺失小鼠引起皮膚早衰的治療作用。方法:將同窩的Bmi-1雜合子(Bmi-1~(+/-))雌雄小鼠進(jìn)行配對(duì),取7周齡正常飲食野生型(wild type,WT)小鼠10只,正常飲食Bmi-1基因敲除(Bmi-1 knock out,BKO)小鼠10只,以及正常飲食添加PQQ的BKO小鼠(PQQ+BKO)10只,利用HE染色、組織化學(xué)、免疫組織化學(xué)及流式細(xì)胞儀檢測(cè)等方法進(jìn)行相關(guān)實(shí)驗(yàn)。結(jié)果:與BKO組小鼠比較,PQQ+BKO組小鼠整體表型部分糾正,毛色光滑,體重增加,生存期明顯延長(zhǎng);PQQ+BKO組小鼠皮膚厚度[(142.65±0.25)μm]與BKO組小鼠[(78.45±0.35)μm]相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);PQQ+BKO組小鼠皮膚總膠原陽(yáng)性面積百分比[(25.64±0.28)%]與BKO組小鼠[(14.87±0.47)%]相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);PQQ+BKO組小鼠皮膚PCNA陽(yáng)性細(xì)胞百分比[(18.54±0.37)%]與BKO組小鼠[(5.85±0.64)%]相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);PQQ+BKO組小鼠皮膚纖維化陽(yáng)性面積百分比[(48.64±0.28)%]與BKO組小鼠[(87.64±0.46)%]相比,差異有統(tǒng)計(jì)學(xué)意義(P0.01);PQQ+BKO組小鼠皮膚的ROS水平(298.17±0.25)較BKO組小鼠(427.80±0.63)顯著下降(P0.01)。結(jié)論:PQQ通過(guò)增加皮膚膠原蛋白、促進(jìn)皮膚細(xì)胞增殖、減少皮膚組織纖維化、清除氧自由基ROS水平等對(duì)Bmi-1缺失所致皮膚早衰發(fā)揮保護(hù)作用。
[Abstract]:Aim: to investigate whether pyrroloquinoline quinone (pyrroloquinoline quinone,PQQ) can inhibit oxidative stress in the treatment of premature skin failure induced by Bmi-1 deficiency in mice. Methods: Bmi-1 heterozygotes (Bmi-1~ (/ -) in the same nest were paired in 10 wild type,WT mice of 7 weeks of normal diet. Bmi-1 knock out, gene knockout of normal diet was carried out in 10 male and female mice. 10 BKO mice and 10 BKO mice fed normal diet with PQQ were tested by HE staining, histochemistry, immunohistochemistry and flow cytometry. Results: compared with BKO group, the whole phenotype of, PQQ BKO group was partly corrected, the coat color was smooth, the weight increased and the survival time was prolonged. The skin thickness of PQQ BKO group [(142.65 鹵0.25) 渭 m] was significantly higher than that of BKO group [(78.45 鹵0.35) 渭 m] (P0.01). The percentage of total collagen positive area in PQQ BKO group [(25.64 鹵0.28)%] was significantly higher than that in BKO group [(14.87 鹵0.47)%] (P0.01). The percentage of PCNA positive cells in skin of PQQ BKO group [(18.54 鹵0.37)%] was significantly higher than that of BKO group [(5.85 鹵0.64)%] (P0.01). The positive area of skin fibrosis in PQQ BKO group [(48.64 鹵0.28)%] was significantly higher than that in BKO group [(87.64 鹵0.46)%] (P0.01). The level of ROS in PQQ BKO group (298.17 鹵0. 25) was significantly lower than that in BKO group (427.80 鹵0. 63) (P0. 01). Conclusion: PQQ has protective effect on premature skin failure caused by Bmi-1 deficiency by increasing skin collagen, promoting skin cell proliferation, reducing skin fibrosis and eliminating ROS level of oxygen free radical.
【作者單位】： 湖南醫(yī)藥學(xué)院口腔醫(yī)學(xué)系;南京醫(yī)科大學(xué)解剖醫(yī)學(xué)系骨與干細(xì)胞研究中心;南京醫(yī)科大學(xué)附屬口腔醫(yī)院口腔種植中心;
【基金】：湖南省教育廳青年科學(xué)研究項(xiàng)目(14B141)
【分類號(hào)】：R965

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