【摘要】：為觀察BAPTA-AM脂質(zhì)體(BA-L)抗重癥急性胰腺炎(SAP)的作用,采用胰膽管逆行注射4%牛黃膽酸鈉(TC)制作大鼠SAP模型.BA-L治療組于造模后立即尾靜脈注射相應(yīng)劑量BA-L,給藥24h后,觀察各組大鼠的死亡情況,檢測各組大鼠血清AMS,LDH,TNF-α水平以及胰腺組織勻漿MDA水平及SOD活性,HE染色觀察胰腺組織病理學(xué)改變.結(jié)果表明:給予BA-L治療后SAP大鼠死亡率明顯降低,血清AMS,LDH,TNF-α水平和胰腺組織勻漿MDA水平顯著降低,SOD活性增高,HE染色組織切片顯示BA-L明顯減輕胰腺組織損傷.表明BA-L能顯著減輕大鼠SAP,為開發(fā)BA-L成臨床SAP治療新藥物提供必要的藥效學(xué)依據(jù).
[Abstract]:To observe the effect of BAPTA-AM liposome (BA-L) on (SAP) in severe acute pancreatitis. The rat model of SAP was established by retrograde injection of 4% sodium bovine cholate (TC) into the pancreatic duct. The death rate of rats in the BA-L treatment group was observed 24 hours after the injection of the corresponding dose of BA-L, into the tail vein immediately after the establishment of the model. The levels of serum AMS,LDH,TNF- 偽, MDA level and SOD activity of pancreatic homogenate were measured in each group. The histopathological changes of pancreas were observed by HE staining. The results showed that the mortality of SAP rats was significantly decreased, the serum AMS,LDH,TNF- 偽 level and pancreatic homogenate MDA level were significantly decreased, and the activity of SOD was increased after BA-L treatment. HE staining showed that BA-L significantly alleviated pancreatic tissue injury. The results showed that BA-L could significantly reduce rat SAP, and provide the necessary pharmacodynamic basis for the development of BA-L as a new drug for clinical SAP treatment.
【作者單位】： 浙江工業(yè)大學(xué)藥學(xué)院;
【分類號】：R965

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 Theodoros E Pavlidis;Efstathios T Pavlidis;Athanasios K Sakantamis;;Advances in prognostic factors in acute pancreatitis:a mini-review[J];Hepatobiliary & Pancreatic Diseases International;2010年05期

2 廖家智;王家，

本文編號：2394941