【摘要】：目的設(shè)計和建立梁氏情境應(yīng)激箱,通過觀察抗焦慮和抗抑郁藥對小鼠行為學(xué)指標(biāo)的影響,評價梁氏情境應(yīng)激箱對小鼠焦慮和(或)抑郁樣情緒反應(yīng)的誘導(dǎo)作用。方法梁氏情境應(yīng)激箱由中央?yún)^(qū)和3個外周臂構(gòu)成。ICR小鼠分別ip給予抗焦慮藥物丁螺環(huán)酮(0.5和1.0 mg·kg~(-1))和抗抑郁藥物米安色林(0.25和0.5 mg·kg~(-1)),觀察小鼠首次進(jìn)入中央?yún)^(qū)的潛伏期、中央?yún)^(qū)停留時間、中央?yún)^(qū)活動路程、外周區(qū)活動路程、區(qū)域間穿梭次數(shù)和總活動度。結(jié)果 1丁螺環(huán)酮0.5和1.0 mg·kg~(-1)明顯縮短小鼠首次進(jìn)入中央?yún)^(qū)的潛伏期,分別縮短了71.9%(P0.05)和77.9%(P0.05);劑量依賴性地增加中央?yún)^(qū)停留時間,各增加了59.5%和73.8%(P0.05);丁螺環(huán)酮0.5和1.0 mg·kg~(-1)均可顯著減少小鼠區(qū)域間穿梭次數(shù)(P0.05),但對其中央?yún)^(qū)活動路程、外周區(qū)活動路程和總活動度無明顯影響;2米安色林0.25和0.5 mg·kg~(-1)明顯縮短小鼠首次進(jìn)入中央?yún)^(qū)的潛伏期,分別縮短了72.4%(P0.05)和82.3%(P0.05);劑量依賴性地增加中央?yún)^(qū)停留時間,各增加了39.1%和43.9%(P0.05);米安色林劑量依賴性地增加中央?yún)^(qū)活動路程(P0.05)及區(qū)域間穿梭次數(shù)(P0.05),但對外周區(qū)活動路程和總活動度無明顯影響。結(jié)論梁氏情境應(yīng)激箱對小鼠焦慮和(或)抑郁樣情緒反應(yīng)具有明確的誘導(dǎo)作用,可作為一個新的焦慮抑郁共病動物模型研究其病理生理機(jī)制,篩選和評價新型抗焦慮和(或)抗抑郁藥物。
[Abstract]:Objective to design and establish the Liang's situational stress box, and to evaluate the inducing effect of Liang's situational stress box on anxiety and / or depression-like emotional response in mice by observing the effect of antianxiety and antidepressant drugs on the behavioral indexes of mice. Methods the stress box consisted of three peripheral arms and the central area. ICR mice were given the antianxiety drug buspirone (0.5 and 1.0 mg kg~ (-1) and the antidepressant mianserin (0.25 and 1.0 mg kg~ (-1), respectively). 0.5 mg kg~ (-1),) The incubation period of entering the central area for the first time, the residence time of the central area, the distance of the activity in the peripheral area, the times of interregional shuttle and the total activity were observed. Results 1 buspirone 0.5 and 1.0 mg kg~ (-1) significantly shortened the latency of entering the central area for the first time by 71.9% (P0.05) and 77.9% (P0.05), respectively. The residence time of central area was increased by 59.5% and 73.8% in a dose-dependent manner (P0.05). Buspirone 0.5 and 1.0 mg kg~ (-1) significantly decreased the number of interregional shuttles in mice (P0.05), but had no significant effect on the distance of activity in the central region, the distance of activity in the peripheral region and the total activity. (2) 0.25 and 0.5 mg kg~ (-1) of mianserine significantly shortened the latency of entering the central area of mice for the first time by 72.4% (P0.05) and 82.3% (P0.05), respectively. The residence time of central area was increased by 39.1% and 43.9% in a dose-dependent manner (P0.05). Mianserin dose-dependently increased the activity distance of central area (P0.05) and the number of interregional shuttle (P0.05), but the distance of activity and the total activity of peripheral area had no significant effect. Conclusion Liang's situational stress box can induce anxiety and / or depression-like emotional response in mice. It can be used as a new animal model to study the pathophysiological mechanism of anxiety and depression syndromes. Screening and evaluation of new anti-anxiety and / or anti-depressants.
【作者單位】： 北京大學(xué)基礎(chǔ)醫(yī)學(xué)院藥理學(xué)系;北京大學(xué)藥學(xué)院分子與細(xì)胞藥理學(xué)系;
【基金】：十二五科技支撐計劃項(xiàng)目(2011BAK04B08)~~
【分類號】：R965

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