【摘要】：目的:探討P-糖蛋白抑制劑醋柴胡小分子水溶性部位(MHE)對甲氨蝶呤藥動學(xué)行為的影響。方法:SD雄性大鼠分別灌胃甲氨蝶呤或甲氨蝶呤聯(lián)合MHE后,于不同時間點眼眶靜脈叢采血,利用20%高氯酸沉淀蛋白法處理血樣,采用高效液相色譜法分析,非隔室模型估算藥代動力學(xué)參數(shù)。結(jié)果:與單用組比較,聯(lián)用組甲氨蝶蛉藥時曲線下面積AUC0-t增加16.6%,AUC0-∞增加33.8%,生物半衰期(t1/2)延長了1.2倍,平均駐留時間(MRT0-t)增加了26.1%,而藥物清除率(CL)下降了20.3%,但2組的藥代動力學(xué)參數(shù)無顯著性差異。結(jié)論:MHE具有增強(qiáng)甲氨蝶呤生物利用度的趨勢,二者聯(lián)合用藥需要關(guān)注毒副反應(yīng)的發(fā)生。
[Abstract]:Aim: to investigate the effect of water soluble fraction (MHE) on the pharmacokinetics of methotrexate (MTX). Methods: after intragastric administration of methotrexate or methotrexate combined with MHE, blood samples were collected from orbital venous plexus at different time points in SD male rats. The blood samples were treated by 20% perchloric acid precipitation protein method and analyzed by high performance liquid chromatography (HPLC). The pharmacokinetic parameters were estimated by non compartment model. Results: compared with the single group, the area under the curve (AUC0-t) increased by 16.6%, AUC0- 鈭，

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