【摘要】：目的:觀察辛伐他汀對腎缺血再灌注損傷后心肌組織的影響及其機(jī)制。方法:隨機(jī)將36只大鼠分為假手術(shù)組、腎缺血再灌注組和辛伐他汀組,每組12只。后2組用夾閉雙側(cè)腎動(dòng)脈的方法復(fù)制腎缺血再灌注損傷模型;辛伐他汀組在造模前給予辛伐他汀(20 mg·kg~(-1)·d~(-1))灌胃,持續(xù)2周。用生化檢查檢測血清肌酐(SCr)、血尿素氮(BUN)、心肌組織丙二醛(MDA)含量及乳酸脫氫酶(LDH)、肌酸激酶(CK)和超氧化物歧化酶(SOD)的活性,并用Western blot法檢測Bcl-2和Bax的表達(dá)水平。結(jié)果:與假手術(shù)組比,缺血再灌注組SCr、BUN和心肌MDA含量均升高(P0.05),心肌LDH和CK活性增強(qiáng)(P0.05),心肌SOD活性明顯下降(P0.05);與缺血再灌注組比較,辛伐他汀組SCr、BUN和心肌MDA的含量降低(P0.05),心肌LDH和CK活性明顯減弱(P0.05),而心肌SOD活性增強(qiáng)(P0.05)。與假手術(shù)組比較,心肌Bcl-2與Bax的蛋白表達(dá)水平在腎缺血再灌注組增多(P0.05);與缺血組相比,Bax表達(dá)在辛伐他汀組明顯降低,而Bcl-2表達(dá)增加(P0.05)。結(jié)論:辛伐他汀對腎缺血再灌注后的心肌有保護(hù)作用,保護(hù)機(jī)制可能與辛伐他汀可以消除自由基、升高Bcl-2蛋白表達(dá)和降低Bax蛋白表達(dá)有一定關(guān)系。
[Abstract]:Aim: to observe the effect and mechanism of simvastatin on myocardial tissue after renal ischemia reperfusion injury. Methods: Thirty-six rats were randomly divided into sham-operation group, renal ischemia reperfusion group and simvastatin group with 12 rats in each group. The model of renal ischemia-reperfusion injury was induced by clamping bilateral renal arteries in the latter two groups, and simvastatin (20 mg kg~ (-1) D1) was administered intragastrically for 2 weeks in simvastatin group. The contents of malondialdehyde (MDA) in serum creatinine (SCr),) and blood urea nitrogen (BUN), and the activities of lactate dehydrogenase (LDH), (LDH), creatine kinase (CK) and superoxide dismutase (SOD) were measured by biochemical examination. The expression levels of Bcl-2 and Bax were detected by Western blot method. Results: compared with sham-operation group, the contents of SCr,BUN and myocardial MDA in ischemia-reperfusion group were increased (P0.05), the activities of myocardial LDH and CK were increased (P0.05), and the activity of myocardial SOD was significantly decreased (P0.05). Compared with ischemia reperfusion group, simvastatin group decreased the content of SCr,BUN and myocardial MDA (P0.05), decreased myocardial LDH and CK activity (P0.05), and increased myocardial SOD activity (P0.05). Compared with sham operation group, the expression of Bcl-2 and Bax in myocardium increased in renal ischemia-reperfusion group (P0.05); compared with ischemic group, the expression of Bax decreased significantly in simvastatin group, while Bcl-2 expression increased (P0.05). Conclusion: simvastatin has protective effect on myocardium after renal ischemia-reperfusion, and the protective mechanism may be related to the action of simvastatin on eliminating free radicals, increasing the expression of Bcl-2 protein and decreasing the expression of Bax protein.
【作者單位】： 石家莊醫(yī)學(xué)高等�？茖W(xué)校;
【基金】：河北省教育廳高等學(xué)�？茖W(xué)技術(shù)指導(dǎo)性研究項(xiàng)目(No.Z2014020)
【分類號】：R965

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