【摘要】：氨茶堿因促進(jìn)內(nèi)源性腎上腺素與去甲腎上腺素釋放而對呼吸道平滑肌有直接松弛作用而用于哮喘病的治療,但其不良反應(yīng)和過敏反應(yīng)常會引起患者死亡。為了考察氨茶堿的對斑馬魚幼魚運(yùn)動失調(diào)的影響,本研究使用不同濃度氨茶堿溶液于24孔板中直接作用于72 hpf孵化的斑馬魚幼魚,定時間記錄各組幼魚的抽搐頻率、心率變化、體形變化等參數(shù),用于評價斑馬魚幼魚運(yùn)動情況。結(jié)果表明,氨茶堿誘導(dǎo)的斑馬魚幼魚開始時運(yùn)動增加,隨后伴有自發(fā)轉(zhuǎn)動,隨著藥物作用時間延長,出現(xiàn)典型的抽搐反應(yīng)而產(chǎn)生嚴(yán)重的顫抖,斑馬魚幼魚出現(xiàn)運(yùn)動失調(diào)輕重程度和快慢呈時間和濃度依賴性。從本實驗結(jié)果推測,氨茶堿應(yīng)慎用或不得用于嬰幼兒和小孩哮喘病的治療,也要慎用于孕期婦女的用藥治療。
[Abstract]:Aminophylline is used in the treatment of asthma because of its direct relaxation effect on respiratory smooth muscle due to promoting the release of endogenous epinephrine and norepinephrine, but its adverse reactions and allergic reactions often cause death in patients. In order to investigate the effect of aminophylline on the movement disorders of juvenile zebrafish, different concentrations of aminophylline solution were directly applied to juvenile zebrafish hatched for 72 hpf in a 24-well plate. The frequency of convulsion and heart rate were recorded at a fixed time. Body shape change and other parameters were used to evaluate the movement of juvenile zebrafish. The results showed that the motility of juvenile zebrafish induced by aminophylline increased at the beginning, followed by spontaneous rotation. Juvenile zebrafish movement imbalance degree and speed are time and concentration dependent. The results suggest that aminophylline should be used with caution or not in the treatment of infant and child asthma, but also in pregnant women.
【作者單位】： 贛南醫(yī)學(xué)院基礎(chǔ)醫(yī)學(xué)院;贛南醫(yī)學(xué)院藥學(xué)院;
【基金】：江西省教育廳科學(xué)技術(shù)研究項目(No.150975)資助
【分類號】：R965

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