【摘要】：目的探討丙戊酸鈉(VPA)對(duì)多發(fā)性骨髓瘤(MM)細(xì)胞株RPMI8226細(xì)胞Notch受體及配體表達(dá)的影響。方法培養(yǎng)RPMI8226細(xì)胞后,分為4組,對(duì)照組(空白)和小中大3個(gè)濃度(VPA 2,4,8 mmol·L-1)實(shí)驗(yàn)組。用不同濃度VPA處理RPMI8226細(xì)胞24,48,72 h,用四甲基偶氮唑藍(lán)比色法檢測(cè)細(xì)胞增殖抑制作用。用反轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)、蛋白免疫印跡方法檢測(cè)細(xì)胞Notch1、jagged1及jagged2 mRNA和Notch1、jagged1及jagged2蛋白在VPA作用后48 h的表達(dá)。結(jié)果 VPA對(duì)RPMI8226細(xì)胞的增殖抑制作用具有明顯的濃度-時(shí)間依賴效應(yīng)。不同濃度VPA作用RPMI8226細(xì)胞48 h,與對(duì)照組相比,Notch1、jagged1、jagged2 mRNA和Notch1、jagged1、jagged2蛋白表達(dá)均明顯降低(P0.05),且隨著VPA濃度的不斷增高,各基因和蛋白的表達(dá)量逐漸降低,3個(gè)濃度VPA組間兩兩比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論 VPA能夠下調(diào)RPMI8226細(xì)胞Notch1受體、jagged1及jagged2配體的表達(dá)水平,這可能是VPA治療多發(fā)性骨髓瘤的作用機(jī)制之一。
[Abstract]:Objective to investigate the effect of sodium valproate (VPA) on the expression of Notch receptor and ligand in multiple myeloma (MM) cell line RPMI8226. Methods RPMI8226 cells were cultured and divided into 4 groups: control group (blank) and small, medium and large concentration (VPA _ 2 + 4 ~ (8) mmol ~ (-1) experimental group. RPMI8226 cells were treated with different concentrations of VPA for 24 ~ 48 ~ 72 h, and the proliferation inhibition was detected by tetramethyl azolyl blue colorimetry. Reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of Notch1,jagged1, jagged2 mRNA, Notch1,jagged1 and jagged2 proteins at 48 h after VPA treatment. Results the inhibitory effect of VPA on the proliferation of RPMI8226 cells was significantly concentration-time dependent. Compared with the control group, the expression of Notch1,jagged1,jagged2 mRNA and Notch1,jagged1,jagged2 protein decreased significantly in RPMI8226 cells treated with different concentrations of VPA for 48 h (P0.05), and with the increasing of VPA concentration, the expression of each gene and protein decreased gradually. There were significant differences among the three VPA groups (P0.05). Conclusion VPA can down-regulate the expression of Notch1 receptor, jagged1 and jagged2 ligand in RPMI8226 cells, which may be one of the mechanisms of VPA in the treatment of multiple myeloma.
【作者單位】： 承德醫(yī)學(xué)院附屬醫(yī)院血液病科;河北中醫(yī)學(xué)院實(shí)驗(yàn)中心;
【基金】：河北省自然科學(xué)基金資助項(xiàng)目(H2013406112)
【分類號(hào)】：R96

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本文編號(hào)：2310797