發(fā)布時間：2018-10-23 15:46

【摘要】：目的:制備葉綠素非離子表面活性劑囊泡(chlorophyll nonionic surfactant vesicles,Chl-NS),并對其理化性質(zhì)進行表征。方法:以非離子表面活性劑脂肪酸山梨坦-80(Span-80)和聚山梨酯-80(Tween-80)復配并結(jié)合膽固醇為膜骨架,采用薄膜超聲分散法制備葉綠素非離子表面活性劑囊泡;利用透射電鏡和Malvern粒度儀分別考察其形態(tài)、粒徑、分散系數(shù)、Zeta電位及穩(wěn)定性;并進一步考察囊泡對葉綠素的包封率以及包載于囊泡中的葉綠素熒光強度的變化。結(jié)果:葉綠素非離子表面活性劑囊泡呈核殼層球形結(jié)構(gòu),大小均勻,平均粒徑為(130.2±10.0)nm,多分散指數(shù)(polydispersity index,PDI)值為(0.240±0.01),Zeta電位為(-30.0±5.0)m V,對葉綠素包封率達到(45.2±2.1)%;葉綠素包載于囊泡后,體系的熒光強度明顯增強且具有較高的穩(wěn)定性。結(jié)論:本實驗制備了穩(wěn)定性高、粒徑均勻、對葉綠素具有良好的包封作用,具有較強近紅外熒光特征的非離子表面活性劑囊泡,該囊泡體系有望應(yīng)用于體內(nèi)成像系統(tǒng)的相關(guān)研究,具有重要臨床意義。
[Abstract]:Aim: to prepare chlorophyll-Nonionic surfactant vesicles (chlorophyll nonionic surfactant vesicles,Chl-NS) and characterize its physicochemical properties. Methods: the surfactant vesicles of chlorophyll Nonionic surfactants were prepared by membrane ultrasonic dispersion with the combination of Nonionic surfactant fatty acid sorbitan-80 (Span-80) and polysorbate 80 (Tween-80) and cholesterol as the membrane skeleton. The morphology, particle size, dispersion coefficient, Zeta potential and stability of the vesicles were investigated by TEM and Malvern particle size analysis respectively, and the changes of the encapsulation efficiency of chlorophyll and the fluorescence intensity of chlorophyll embedded in the vesicles were further investigated. Results: chlorophyll Nonionic surfactant vesicles were core-shell spherical and uniform in size. The average diameter of the vesicles was (130.2 鹵10.0) nm, polydispersity index (polydispersity index,PDI) was (0.240 鹵0.0l), Zeta potential was (-30.0 鹵5.0) m V, the encapsulation efficiency of chlorophyll was (45.2 鹵2.1)%, and the chlorophyll encapsulation rate was (45.2 鹵2.1)%. The fluorescence intensity of the system is obviously enhanced and the system has high stability. Conclusion: Nonionic surfactant vesicles with high stability, uniform particle size, good encapsulation to chlorophyll and strong near infrared fluorescence characteristics were prepared in this experiment. The vesicle system is expected to be used in the study of imaging system in vivo, which has important clinical significance.
【作者單位】： 濟南大學山東省醫(yī)學科學院醫(yī)學與生命科學學院;濟寧醫(yī)學院藥學院;
【分類號】：R943

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