【摘要】：目的探討維甲酸類藥物對(duì)表皮角阮細(xì)胞中水孔蛋白通道(AQP)3表達(dá)的影響。方法異維A酸、阿維A、阿達(dá)帕林處理角朊細(xì)胞的體外培養(yǎng)模型(Ha Ca T),采用免疫組化、RT-PCR、Western印跡分子生物學(xué)方法檢測(cè)不同濃度(0.000、0.001、0.010、0.060、0.100 mg/ml)、不同作用時(shí)間(0、6、12、24、48 h)及不同干預(yù)因素下對(duì)Ha Ca T中AQP3含量的變化。結(jié)果 1濃度為0.010 mg/ml時(shí),三組Ha Ca T中AQP3的表達(dá)均高于其他濃度(P0.05);20.010 mg/ml濃度的異維A酸、阿維A、阿達(dá)帕林作用12 h,三組Ha Ca T中AQP3的表達(dá)異維A酸組阿維A組阿達(dá)帕林組,組間比較差異顯著(P0.05)。結(jié)論維甲酸可調(diào)節(jié)Ha Ca T中AQP3的表達(dá),0.010 mg/ml濃度的異維A酸組12 h的時(shí)候?qū)a Ca T中AQP3表達(dá)的上調(diào)作用最明顯。
[Abstract]:Objective to investigate the effect of retinoic acid on the expression of aquaporin channel (AQP) 3 in keratokeratoin cells. Methods Isoretinoic acid, (Ha Ca T), an in vitro culture model of keratinocytes treated with Avera and Adalparine, was used to detect the changes of AQP3 content in Ha Ca T at different concentrations (0. 000,0. 001P0. 01010, 0. 060,0. 100 mg/ml) and different time (0. 060? 2448h) and different intervention factors by immunohistochemistry and RT-PCR,Western blot molecular biology. Results (1) at 0.010 mg/ml, the expression of AQP3 in Ha Ca T of the three groups was higher than that of the other groups (P0.05), the expression of AQP3 in Ha Ca T of the three groups was increased after 12 h exposure to isoretinoic acid, avea and adalapalin at the concentration of 20.010 mg/ml, and the expression of AQP3 in the Ha Ca T of the three groups was higher than that of the control group (P0.05). There was significant difference between the two groups (P0.05). Conclusion Retinoic acid can regulate the expression of AQP3 in Ha Ca T, and the up-regulation of AQP3 expression in Ha Ca T is most obvious in 0.010 mg/ml isoretinoic acid group at 12 h.
【作者單位】： 江西中醫(yī)藥高等專科學(xué)校人體解剖學(xué)教研室;
【分類號(hào)】：R96

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 楊娟;張錫寶;;維甲酸受體及其作用機(jī)制研究進(jìn)展[J];皮膚性病診療學(xué)雜志;2014年05期

2 王滬凱;;維甲酸藥物對(duì)皮膚病的治療探討[J];中國(guó)藥物經(jīng)濟(jì)學(xué);2012年03期

3 徐之涵;潘q，

本文編號(hào)：2283079