【摘要】：目的探討白介素(IL)-17單克隆抗體(m Ab)對博萊霉素所致大鼠肺纖維化的保護作用及相關(guān)機制。方法將♂SD大鼠75只隨機分為正常對照組、假手術(shù)組、模型組、非特異性Ig G組、IL-17 m Ab組,各組15只,后3組大鼠予以博萊霉素A5氣管內(nèi)注入構(gòu)建肺纖維化模型,而假手術(shù)組給予等量生理鹽水氣管內(nèi)注入。后2組于d 7、14、21分別給予非特異性IgG、IL-17 mAb尾靜脈注射,其余3組給予等量生理鹽水尾靜脈注射。所有大鼠d 28處死,留取肺組織,行HE和Masson染色,通過酶聯(lián)免疫吸附法(ELISA)測定肺組織IL-17、IL-6、腫瘤壞死因子-α(TNF-α)含量,Western blot分析肺組織胞核核因子-κB(NF-κB)p65以及細胞Ⅰ型膠原(ColⅠ)、Ⅲ型膠原(ColⅢ)蛋白表達情況,熒光實時定量PCR檢測肺組織ColⅠ、ColⅢmRNA表達水平,分離血清,采用ELISA檢測血清Ⅰ型前膠原羧基端前肽(PICP)、Ⅲ型前膠原氨基端前肽(PIIINP)濃度。結(jié)果 IL-17m Ab組肺泡炎癥和肺纖維化程度明顯輕于模型組及非特異性Ig G組(P0.01)。模型組和非特異性Ig G組肺組織IL-17、IL-6、TNF-α含量、胞核NF-κB p65蛋白表達、ColⅠ、ColⅢmRNA和蛋白表達以及血清PICP、PIIINP濃度較正常對照組及假手術(shù)組明顯升高(P0.01)。經(jīng)IL-17 m Ab處理后,上述指標相比模型組和非特異性IgG組明顯降低(P0.01)。但非特異性Ig G組與模型組以及假手術(shù)組與正常對照組上述指標比較無差異(P0.05)。結(jié)論 IL-17 mAb通過下調(diào)NF-κB表達,抑制肺組織炎癥反應及減少膠原蛋白合成,對肺纖維化大鼠產(chǎn)生保護作用。
[Abstract]:Objective to investigate the protective effect of interleukin (IL)-17 monoclonal antibody (m Ab) on bleomycin-induced pulmonary fibrosis in rats. Methods Seventy-five SD rats were randomly divided into normal control group, sham operation group, model group, non-specific Ig G group, IL-17 m Ab group and 15 rats in each group. In the sham operation group, the same amount of saline was injected into the trachea. The latter two groups were injected with nonspecific IgG,IL-17 mAb caudal vein on day 7, 14 and 21, respectively, and the other three groups were given the same amount of normal saline tail vein injection. All the rats were killed on d28, the lung tissue was taken and stained with HE and Masson. The expression of nuclear factor- 魏 B (NF- 魏 B) p65 and collagen 鈪，

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