【摘要】：目的探索托芬那酸對大鼠大腸癌的預(yù)防作用。方法選擇Wistar清潔級大鼠作為實驗對象,隨機數(shù)字表法隨機分為陰性對照組、二甲基肼(dimethylhydrazine,DMH)誘癌組、托芬那酸(tolfenamic acid,TA)對照組和實驗組,每組40只大鼠,陰性對照組給予生理鹽水皮下注射;DMH誘癌組給予二甲基肼皮下注射建立大鼠大腸癌模型;托芬那酸對照組單純給予托芬那酸注射液;實驗組分為0.1和0.2 ml/kg托芬那酸組,給予二甲基肼溶液皮下注射的同時分別給予0.1和0.2 ml/kg托芬那酸注射液,腹腔注射,1次/d,連續(xù)32周,大鼠分別于12和32周進(jìn)行解剖,觀察大鼠的一般狀態(tài)以及腫瘤發(fā)生情況,并對32周的托芬那酸對照組大鼠的形態(tài)以及毒性作用進(jìn)行評價。結(jié)果 12周時,各組大鼠均無腫瘤產(chǎn)生;32周時,DMH誘癌組20只,19只產(chǎn)生腫瘤,發(fā)生率為95.0%;0.1 ml/kg托芬那酸組20只大鼠,9只產(chǎn)生腫瘤,發(fā)生率為45.0%;0.2 ml/kg托芬那酸組20只大鼠,3只產(chǎn)生腫瘤,發(fā)生率為15.0%,與DMH誘癌組相比差異具有統(tǒng)計學(xué)意義(P0.05);陰性對照組和托芬那酸對照組所有大鼠在實驗32周時均未產(chǎn)生腫瘤。對腫瘤大小進(jìn)行測量,發(fā)現(xiàn)實驗組的腫瘤直徑為1~7 mm,普遍低于DMH誘癌組。托芬那酸對照組所有大鼠一般狀態(tài)良好,給藥32周未出現(xiàn)死亡,體質(zhì)量與陰性對照組對比差異無統(tǒng)計學(xué)意義,出現(xiàn)2例大鼠腹腔給藥30 min后出現(xiàn)身體震顫、尾巴隆起癥狀,1例大鼠發(fā)生十二指腸潰瘍,肉眼觀察肝臟、腎臟、心臟等重要組織器官,未發(fā)現(xiàn)任何增生組織。結(jié)論托芬那酸對二甲基肼誘發(fā)的大鼠大腸癌具有拮抗作用。
[Abstract]:Objective to explore the preventive effect of topenaric acid on colorectal cancer in rats. Methods Wistar clean grade rats were randomly divided into three groups: negative control group, dimethylhydrazine (dimethylhydrazine,DMH) induced cancer group, Topenaric acid (tolfenamic acid,TA) control group and experimental group, with 40 rats in each group. The negative control group was given normal saline subcutaneous injection of DMH-induced carcinoma group to establish the rat model of colorectal cancer by subcutaneous injection of dimethylhydrazine; the tofenac control group was given only topenaric acid injection; the experimental group was divided into 0. 1 and 0. 2 ml/kg tofenac groups. Dimethylhydrazine solution was injected subcutaneously with 0. 1 and 0. 2 ml/kg topenic acid injection respectively. The rats were dissected at 12 and 32 weeks to observe the general state and tumorigenesis of the rats. The morphologic and toxic effects of tofenac control group at 32 weeks were evaluated. Results at the 12th week, no tumor was produced in all groups. At 32 weeks, there were 19 carcinomas in the DMH-induced carcinomas group (n = 19). The incidence of tumor was 95.0 ml/kg / 0. 1 ml/kg topenaric acid group (20 rats) and 9 rats (n = 9). The incidence rate was 45. 0 ml/kg / 0. 2 ml/kg topenic acid group (20 rats) and 3 rats (n = 3). The incidence rate was 15.0 and the difference was statistically significant compared with DMH induced cancer group (P0.05). All the rats in the negative control group and topenaric acid control group did not produce tumor at 32 weeks of experiment. It was found that the tumor diameter of the experimental group was 1 ~ 7 mm, which was lower than that of the DMH induced cancer group. All the rats in the topenaric acid control group were generally in good condition, and no death occurred in 32 weeks after administration, and there was no significant difference in body mass between the two groups. There were 2 rats with tremor after 30 min of intraperitoneal administration. Duodenal ulcer was found in one rat with symptoms of tail protuberance. The liver, kidney, heart and other important tissues and organs were observed with the naked eye. No proliferative tissue was found. Conclusion Tofenaric acid has an antagonistic effect on rat colorectal cancer induced by dimethylhydrazine.
【作者單位】： 四川省三臺縣人民醫(yī)院;泰州海達(dá)醫(yī)藥科技研究所;
【分類號】：R965

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本文編號：2237563