【摘要】：本研究采用肉湯微量稀釋法對阿魏精油的抗菌活性進行了研究,同時使用紫色桿菌作為細菌模型對阿魏精油的群體抑制劑活力進行了評估。結果表明:阿魏可作為與食品有關微生物和群體感應的抑制劑。阿魏精油的最小抑菌濃度(MIC)值為0.25 mg/m L,可以抑制所有被測細菌的生長。對于阪崎腸桿菌/大腸桿菌/鼠傷寒沙門菌/李斯特菌,其最小殺菌濃度(MBC)值分別為0.25、0.50、0.48、0.48 mg/m L。此外,含濃度為6.25和12.5 mg/m L的阿魏精油紙片顯著的抑制紫色桿菌(Chromobacterium violaceum)色素的產生,但不影響紫色桿菌的生長。當濃度被提升到25 mg/m L,既抑制色素的產生也抑制細菌的生長。同樣地,所有濃度的阿魏精油抑制了紫色桿菌素的產生,即使最低濃度的阿魏精油0.13 mg/m L,也使色素產物減少38%以上;濃度為0.13、0.50和1.0 mg/m L的阿魏精油不影響紫色桿菌的生存能力,只有最高濃度2.0 mg/m L抑制了細菌的生長,在培養(yǎng)后使細菌數(shù)量減少1.37 log CFU/m L。這些結果表明阿魏精油作為一種新型的群體感應抑制劑可有效影響紫色桿菌的群體感應機制。
[Abstract]:The antimicrobial activity of essential oil of Ferula ferulae was studied by broth microdilution method, and the activity of population inhibitor of essential oil of Ferula was evaluated by using purple bacillus as a bacterial model. The results showed that Ferula could be used as a food-related microbial and population-sensitive inhibitor. The minimum inhibitory concentration (MIC) of essential oil was 0.25 mg/m / L, which inhibited the growth of all tested bacteria. For Enterobacter sakazakii / Escherichia coli / Salmonella typhimurium / Listeria, the minimum bactericidal concentration (MBC) was 0.250.50,0.48 mg/m / 0.48 mg/m / L, respectively. In addition, the essential oil paper containing 6.25 and 12.5 mg/m / L of ferulic oil significantly inhibited the production of (Chromobacterium violaceum) pigment from purple bacillus, but did not affect the growth of purple bacillus. When the concentration was raised to 25 mg/m L, both the production of pigment and the growth of bacteria were inhibited. Similarly, all concentrations of ferulic essential oil inhibited the production of purple bacterein. Even the lowest concentration of ferulic essential oil (0.13 mg/m / L) reduced the pigment product by more than 38%, while the concentration of 0.130.50 and 1.0 mg/m / L of ferulic essential oil did not affect the viability of purple bacillus. Only the highest concentration of 2.0 mg/m / L inhibited the growth of bacteria and reduced the number of bacteria by 1.37 log CFU/m / L. These results suggest that ferulic essential oil, as a new type of colony sensing inhibitor, can effectively affect the colony sensing mechanism of purple bacillus.
【作者單位】： 福建農林大學食品科學學院;
【基金】：國家自然科學基金資助項目(31101327) 國家青年科學基金項目資助項目(31401597) 福建省自然科學項目基金資助項目(2013J01084)
【分類號】：R915

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