【摘要】：目的：觀察雷公藤多苷對雌性小鼠性腺、卵巢功能的影響；探討曲普瑞林、六子方對雷公藤多苷致雌性小鼠卵巢功能受損的保護作用；尋求切實有效的應(yīng)用雷公藤時保護卵巢功能的方案。方法：篩選出動情周期正常者40只為受試動物,隨機分為4組,每組10只：A組空白對照組：每日灌胃生理鹽水0.35ml,每日1次,連續(xù)11周；B組雷公藤多苷組：第8天起用雷公藤多苷溶液灌胃0.35ml,每日1次,連續(xù)10周；C組雷公藤多苷+曲普瑞林組：每天O.lmg/kg皮下注射曲普瑞林注射液,每日1次,連續(xù)注射11周；第8天起用雷公藤多苷溶液灌胃0.35ml,每日1次,連續(xù)10周；D組雷公藤多苷+六子方：第8天起7AM用雷公藤多苷溶液灌胃0.35ml,每日1次,連續(xù)10周；7PM用六子方溶液灌胃0.35m1,每日1次,連續(xù)10周；第11周各組均處死,檢測血AMH、FSH、LH、E2,計算卵巢指數(shù),作卵巢組織形態(tài)學(xué)觀察。篩選最佳方案。結(jié)果：曲普瑞林與雷公藤配伍使用,小鼠活動靈敏；體重增加；FSH、LH降低,AMH、 E2升高；卵巢中卵泡數(shù)、成熟卵泡數(shù)均較多。小鼠使用六子方后一般情況良好；動情周期延長;小鼠卵巢指數(shù)及卵泡數(shù)量明顯增多；小鼠血清FSH、LH有升高趨勢,血清E2與AMH水平高。結(jié)論：1、雷公藤多苷可造成雌性小鼠性腺、卵巢功能損傷；2、曲普瑞林、六子方對雷公藤多苷致雌性小鼠性腺、卵巢功能受損有保護作用。
[Abstract]:Objective: to observe the effect of tripterygium wilfordii polyglycoside on gonadal and ovarian function in female mice, and to explore the protective effect of tripterygium tripterygii polyglycoside on ovarian function damage in female mice induced by tripterygium wilfordii. To find a practical and effective way to protect ovarian function when using Tripterygium wilfordii. Methods: 40 subjects with normal estrous cycle were randomly divided into 4 groups: control group (n = 10): normal saline 0.35 ml daily, once a day for 11 weeks; Group B was treated with tripterygium wilfordii polyglycoside 0.35 ml by gavage on the 8th day, once a day for 10 weeks. Group C was given tripterygium polyglycoside triplatin for 10 weeks. The injection of tripterygium tripterygii was subcutaneously injected daily once a day for 11 weeks. From the 8th day, 7AM was gavaged with Tripterygium wilfordii polyglycoside solution 0.35 ml, once a day, for 10 weeks. Group D was treated with Tripterygium wilfordii polyglycoside for 10 weeks. From the 8th day on, 7AM was gavaged with tripterygium wilfordii polyglycoside solution 0.35 ml, once a day for 10 weeks. 7PM was perfused with Liuzi Fang solution once a day for 10 weeks, and all the groups were killed at the 11th week. The serum AMHH FSHSHLHHU E2 was detected, the ovarian index was calculated and the ovarian histomorphology was observed. Select the best scheme. Results: when combined with Tripterygium wilfordii, the mice were sensitive to activity, FSH LH decreased AMH and E2 increased, follicles and mature follicles were more in ovary. The ovarium index and the number of follicles in mice were obviously increased. The serum FSH LH and E 2 and AMH levels were higher in mice. ConclusionTwain 1, Tripterygium wilfordii polyglycoside can cause sexual gland, ovarian function injury, triplatin and liuzi prescription in female mice. It has protective effect on female sexual gland induced by tripterygium wilfordii polyglycoside.
【學(xué)位授予單位】：浙江中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2015
【分類號】：R965;R285.5
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本文編號：2196245