【摘要】：光動(dòng)力療法(PDT)是一種區(qū)別于傳統(tǒng)治療腫瘤的方法,其基本原理是腫瘤患者注射光敏劑藥物后,藥物能夠靶向富集于腫瘤組織周圍,在一定波長(zhǎng)的光照條件下,光敏劑藥物吸收光能后激發(fā)與腫瘤周圍組織氧作用,生成活性較高的單線態(tài)氧,單線態(tài)氧最終誘導(dǎo)腫瘤細(xì)胞死亡,達(dá)到治療腫瘤目的。由于光敏劑具有腫瘤靶向性差、分子之間易聚集等缺陷限制了其在PDT中的應(yīng)用。為了解決這些問題,本文開展具有軸向性、生物靶向性錫卟啉類光敏劑的研究。本文首先采用Adler法制備了四苯基卟啉,通過吡啶法得到軸向四苯基錫卟啉,Sn Cl2·2H2O提供金屬配體,然后接枝丙二酸和乙�；被咸烟�;其次用濃H2SO4為磺化劑磺化四苯基卟啉得到四(4-磺酸基苯基)卟啉,通過甲醇法得到金屬錫配位卟啉,接枝透明質(zhì)酸得到軸向四(4-磺酸基苯基)錫卟啉-透明質(zhì)酸。這兩種軸向卟啉其結(jié)構(gòu)通過核磁共振氫譜(1H NMR)、紫外可見光譜(UV-Vis)和紅外光譜(FT-IR)等進(jìn)行表征。最后,以1,3-二苯基異苯并呋喃(DPBF)作為單線態(tài)氧的捕獲劑測(cè)定兩種物質(zhì)的單線態(tài)氧量子產(chǎn)率。結(jié)果表明,這兩種卟啉類光敏劑能夠產(chǎn)生較高的單線態(tài)氧,產(chǎn)率分別為0.68和0.73。說明軸向配位可以有效的解決卟啉分子間的自聚,保證了單線態(tài)氧的產(chǎn)率。所以,在生物醫(yī)學(xué)領(lǐng)域里具有潛在的應(yīng)用價(jià)值。
[Abstract]:Photodynamic therapy (PDT) is different from traditional methods in the treatment of tumor. Its basic principle is that after the tumor patients are injected with Guang Min, the drug can be targeted around the tumor tissue and be exposed to light at a certain wavelength. The drug of Guang Min stimulates the action of oxygen around the tumor tissue after absorbing the light energy and produces the highly active singlet oxygen which eventually induces the death of tumor cells and achieves the purpose of treating tumor. The application of Guang Min in PDT is limited due to its poor tumor targeting and easy aggregation among molecules. In order to solve these problems, the axial and biologically targeted tin porphyrin (Guang Min) agents have been studied in this paper. In this paper, tetraphenylporphyrin was prepared by Adler method. The tetraphenyltin porphyrin Sn Cl2 2H2O was prepared by pyridine method and then grafted with malonic acid and acetylated glucosamine. Tetra (4-sulfophenyl) porphyrin was obtained by sulfonation of tetraphenylporphyrins with concentrated H2SO4 as sulfonation agent. Metalloporphyrins were synthesized by methanol method, and tetra-( 4-sulfophenyl) tin porphyrin-hyaluronic acid was grafted onto hyaluronic acid to obtain tetra- (4-sulfophenyl) tin porphyrin-hyaluronic acid. The structures of these two kinds of porphyrins were characterized by 1H NMR), UV-Vis (UV-Vis) and IR (FT-IR) spectra. Finally, the single-line oxygen yields of two kinds of substances were determined by using 1 ~ 3- diphenyl isobenzofuran (DPBF) as the trapping agent of singlet oxygen. The results show that the two porphyrin Guang Min agents can produce higher singlet oxygen with the yields of 0.68 and 0.73, respectively. The results show that axial coordination can effectively solve the self-polymerization of porphyrin molecules and ensure the yield of singlet oxygen. Therefore, it has potential application value in biomedical field.
【學(xué)位授予單位】：長(zhǎng)春理工大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R943

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