【摘要】：目的:蜂膠是一種蜜蜂生產(chǎn)的被廣泛使用天然產(chǎn)品,具有消炎、殺菌、抑制腫瘤等作用為人類(lèi)的健康做出很大貢獻(xiàn)。近年來(lái)癌癥已成為危害人體健康的首要疾病,目前其主要治療途徑是放療,但放療副作用很大,能殺死一部分正常的細(xì)胞。蜂膠作為一種具有抗腫瘤生物學(xué)活性的天然產(chǎn)物,可誘使癌細(xì)胞凋亡對(duì)正常細(xì)胞沒(méi)有影響。本課題通過(guò)正交試驗(yàn)探究蜂膠醇提物黃酮類(lèi)化合物的優(yōu)化方案;觀(guān)察東北黑蜂蜂膠醇提取物(EEP)對(duì)荷瘤小鼠免疫活性的影響和腫瘤抑制率,探討EEP的抗腫瘤活性。方法:制備H22模型小鼠,將蜂膠用乙醇提取做正交試驗(yàn)優(yōu)化實(shí)驗(yàn)方案再對(duì)東北黑蜂蜂膠進(jìn)行提取,進(jìn)行體內(nèi)實(shí)驗(yàn)。碳廓清試驗(yàn)和抗腫瘤實(shí)驗(yàn),把小鼠分成陰性對(duì)照組、蜂膠高劑量組組、蜂膠中劑量組、蜂膠低劑量組、環(huán)磷酰胺組五組,連續(xù)給藥7天,稱(chēng)體重。注射印度墨汁,測(cè)定碳廓清的吞噬指數(shù)。最后一次給藥后脫頸處死解剖取臟器,再精確稱(chēng)量其重,計(jì)算黑蜂蜂膠提取物腫瘤抑制率。結(jié)果:乙醇提取東北黑蜂蜂膠黃酮化合物的優(yōu)化方案是溫度為常溫,固液比為1:10,乙醇濃度為90%,時(shí)間為12 h;將碳廓清試驗(yàn)中的黑蜂蜂膠劑量組的吞噬指數(shù)與陰性對(duì)照組做比較,具有統(tǒng)計(jì)學(xué)意義(P0.05);抗腫瘤實(shí)驗(yàn)中的黑蜂蜂膠的三個(gè)組的瘤重分別與陰性對(duì)照組作比較,有統(tǒng)計(jì)學(xué)意義。結(jié)論:優(yōu)化后的方案使黃酮提取成本降低,符合實(shí)際需要;碳廓清實(shí)驗(yàn)說(shuō)明東北黑蜂蜂膠醇提取物能夠提高H22小鼠的免疫活性;抗腫瘤實(shí)驗(yàn)說(shuō)明東北黑蜂蜂膠醇提取物對(duì)實(shí)體瘤的生長(zhǎng)在一定程度上有很強(qiáng)的抑制作用。綜上實(shí)驗(yàn)表明東北黑蜂蜂膠的乙醇提取物黃酮類(lèi)化合物抗腫瘤活性顯著。
[Abstract]:Objective: propolis is a widely used natural product produced by honeybees. It has the functions of anti-inflammatory, bactericidal and tumor inhibition, and makes a great contribution to human health. In recent years, cancer has become the primary disease that endangers human health. At present, radiotherapy is the main treatment way, but the side effects of radiotherapy are very big, which can kill some normal cells. Propolis, as a natural product with anti-tumor biological activity, can induce cancer cell apoptosis to have no effect on normal cells. In this paper, the optimum scheme of flavonoids extracted from propolis alcohol was studied by orthogonal experiment, and the effect of (EEP) on the immune activity and tumor inhibition rate of mice bearing tumor was observed, and the anti-tumor activity of EEP was discussed. Methods: H22 model mice were prepared. The ethanol extraction of propolis was optimized by orthogonal experiment. The mice were divided into negative control group, high dose propolis group, middle dose propolis group, low dose propolis group and cyclophosphamide group. The phagocytic index of carbon clearance was measured by injection of Indian ink. After the last administration, the organs were dissected and weighed accurately, and the tumor inhibition rate of the extract of Propolis was calculated. Results: the optimum scheme of ethanol extraction was as follows: the temperature was normal temperature, the ratio of solid to liquid was 1: 10, the concentration of ethanol was 90 and the time was 12 hours. The phagocytic index of the dose group of propolis in carbon clearance test was compared with that of the negative control group. There was statistical significance (P0.05); the tumor weight of the three groups of black bee propolis in the anti-tumor experiment was compared with the negative control group, which had statistical significance. Conclusion: the optimized scheme can reduce the cost of flavonoids extraction and meet the practical needs, and the carbon clearance experiment shows that the alcohol extract of Propolis can improve the immune activity of H22 mice. The antitumor experiment showed that the alcohol extract of Propolis had a strong inhibitory effect on the growth of solid tumor to a certain extent. The experimental results showed that the flavonoids from the ethanol extract of Propolis were significant in anti-tumor activity.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：TQ464;R965

【相似文獻(xiàn)】

相關(guān)博士學(xué)位論文 前2條

1 姜玉;東北地區(qū)人口遷移流動(dòng)及其影響研究[D];吉林大學(xué);2017年

2 王洪橋;東北地區(qū)旅游空間格局演化及機(jī)制研究[D];東北師范大學(xué);2017年

相關(guān)碩士學(xué)位論文 前10條

1 孫小英;東北黑蜂蜂膠黃酮提取工藝優(yōu)化及抗腫瘤活性研究[D];佳木斯大學(xué);2017年

2 王瑤;淺談黑土地民俗儀式在東北舞蹈中的創(chuàng)作[D];吉林藝術(shù)學(xué)院;2017年

3 邊彤彤;東北地區(qū)拉丁舞培訓(xùn)機(jī)構(gòu)經(jīng)營(yíng)現(xiàn)狀與對(duì)策研究[D];哈爾濱體育學(xué)院;2017年

4 姚德華;東北淪陷時(shí)期日偽喇嘛教政策研究[D];遼寧大學(xué);2017年

5 陳小明;新世紀(jì)以來(lái)東北戲劇對(duì)民間資源的利用[D];遼寧師范大學(xué);2017年

6 陳浩;中國(guó)東北地區(qū)水稻時(shí)空分布變化及其驅(qū)動(dòng)因素分析[D];中國(guó)農(nóng)業(yè)科學(xué)院;2017年

7 邵巖;東北地區(qū)近300年來(lái)社會(huì)變遷下的耕地變化分析[D];沈陽(yáng)農(nóng)業(yè)大學(xué);2017年

8 馮祥赫;日俄戰(zhàn)后清政府挽救東北危局的聯(lián)美外交[D];遼寧師范大學(xué);2017年

9 王海鳳;抗戰(zhàn)時(shí)期國(guó)民政府的東北學(xué)生教育救濟(jì)研究[D];華中師范大學(xué);2017年

10 魏明友;東北地區(qū)外貿(mào)單一窗口建設(shè)與管理研究[D];哈爾濱工程大學(xué);2014年

，

本文編號(hào)：2162302