本文選題：1-甲基乙內(nèi)酰脲 + 生長激素��； 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的： 探索1-甲基乙內(nèi)酰脲能否降低生長激素水平，是否具有生長抑素類似物的功能，為非肽類生長抑素的研發(fā)提供實(shí)驗(yàn)基礎(chǔ)及理論依據(jù)。 方法： 實(shí)驗(yàn)對象：雌性SD大鼠80只，體重230-250g。分組：將80只雌性SD大鼠按給藥途徑不同隨機(jī)分為靜脈組(V1-V5)和腹腔組（P1-P5）10組，每個(gè)大組設(shè)空白對照組（V1組、P1組）、造模對照組（V2組、P2組）、實(shí)驗(yàn)組（V3-V5組和P3-P5組），每組SD大鼠8只。處理：空白對照組（V1組、P1組）予以生理鹽水0.5ml腹腔注射。造模對照組（V2組、P2組）予以10mg/ml苯巴比妥0.5ml腹腔注射造模。實(shí)驗(yàn)組先予以10mg/ml苯巴比妥0.5ml腹腔注射造模，10分鐘后再對各個(gè)實(shí)驗(yàn)組按照不同給藥途徑及劑量給藥：V3組、P3組注射10mg的1-甲基乙內(nèi)酰脲0.5ml，V4組、P4組注射1mg的1-甲基乙內(nèi)酰脲0.5ml，V5組、P5組注射0.1mg的1-甲基乙內(nèi)酰脲0.5ml；空白對照組（V1組、P1組）、造模對照組（V2組、P2組）則分別給予PBS0.5ml。取樣：各組均在注射生理鹽水或者苯巴比妥造模后20分鐘、30分鐘、40分鐘時(shí)由尾靜脈取血，離心取上層血清。檢測：按大鼠生長激素試劑盒說明做血清ELISA檢測生長激素水平，對比各組之間生長激素水平變化。 結(jié)果： 1.造模對照組較空白對照組的生長激素水平增高（P0.01）； 2.各個(gè)實(shí)驗(yàn)組較造模對照組生長激素水平降低（P0.01）； 3.靜脈實(shí)驗(yàn)組（V3-V5組）組間比較：靜脈注射1-甲基乙內(nèi)酰脲10mg的V3組生長激素水平最低（P0.05），對SD大鼠生長激素的抑制效果最明顯。 4.腹腔實(shí)驗(yàn)組（P3-P5組）組間比較：腹腔注射1-甲基乙內(nèi)酰脲10mg的P3組生長激素水平最低（P0.05），，對SD大鼠生長激素的抑制效果最明顯。 5.相同劑量的靜脈實(shí)驗(yàn)組與腹腔實(shí)驗(yàn)組生長激素比較：靜脈實(shí)驗(yàn)組的生長激素激素水平較腹腔實(shí)驗(yàn)組的生長激素水平低，V3組在用藥后10分鐘、20分鐘（即造模后20分鐘、30分鐘）的生長激素水平低于P3組（P0.05），V4組在用藥后10分鐘、20分鐘、30分鐘（即造模后20分鐘、30分鐘、40分鐘）的生長激素水平低于P4組（P0.05）。 6.造模組在造模后30分鐘的生長激素水平最高，實(shí)驗(yàn)組（V3-V4組、P3-P5組）在用藥后20分鐘（即造模后30分鐘）的生長激素水平最低，V3組、P3組用藥后20分鐘（即造模后30分鐘）的生長激素水平低于用藥后10分鐘和30分鐘（即造模后20分鐘和40分鐘）（P0.05），P4組、P5組用藥后20分鐘（即造模后30分鐘）的生長激素水平低于用藥后30分鐘（即造模后40分鐘）（P0.05）。 結(jié)論： 1.1-甲基乙內(nèi)酰脲能降低大鼠生長激素水平，在0.1mg~10mg之間有劑量依賴性，可能是一種新型的非肽類生長抑素。 2.1-甲基乙內(nèi)酰脲注射后20分鐘對大鼠生長激素的抑制效果最佳。 3.1-甲基乙內(nèi)酰脲對大鼠生長激素的抑制效果靜脈途徑優(yōu)于腹腔途徑。
[Abstract]:Aim: to explore whether 1-methyl-acetonide can reduce growth hormone level and whether it has the function of somatostatin analogues, and provide experimental basis and theoretical basis for the development of non-peptide somatostatin. Methods: 80 female SD rats, weighing 230-250 g. Group: 80 female Sprague-Dawley rats were randomly divided into intravenous group (V1-V5) and peritoneal group (P1-P5). Each group was divided into blank control group (V1 group), model control group (V2 group P2 group), experimental group (V3-V5 group and P3-P5 group) with 8 SD rats in each group. Treatment: the blank control group (group V _ 1, P _ 1) was injected with normal saline 0.5ml intraperitoneally. 10mg/ml phenobarbital 0.5ml was injected intraperitoneally to model control group (group V 2, P 2). The experimental group was given 10mg/ml phenobarbital 0.5ml intraperitoneally for 10 minutes, then each experimental group was injected with 1-methyl-ethylglycolide (1-methylacetol urea-0.5ml) of 10mg in the P3 group according to different routes and doses of the drug, and then the 1-methylethylethyl of 1mg was injected into the P4 group. The 1-methyl-glycolylurea (1-methyl-glycolide) of 0.1mg was injected into the P5 group (0.5 ml) with 0.5 ml lactol urea (V5). The blank control group (V1 group) and the model control group (V2 group P 2 group) were given PBS 0.5 ml. Sampling: blood was collected from caudal vein 20 minutes after injection of normal saline or phenobarbital for 40 minutes after injection of phenobarbital. Detection: according to the instructions of rat growth hormone kit, serum Elisa was used to detect growth hormone level, and the changes of growth hormone level between groups were compared. Results: 1. The growth hormone level in the model control group was higher than that in the blank control group (P0.01); 2. The growth hormone level of each experimental group was lower than that of model control group (P0.01). Comparison of intravenous experimental group (V3-V5 group): intravenous injection of 1-methylacetol urea 10mg in V3 group had the lowest growth hormone level (P0.05), and the inhibitory effect on growth hormone in SD rats was the most obvious. 4. Comparison between the experimental group (P3-P5): the growth hormone level in P3 group was the lowest (P0.05), and the inhibitory effect on growth hormone in SD rats was the most obvious. 5. 5. Comparison of growth hormone levels between intravenous and celiac groups at the same dose: growth hormone levels in intravenous experimental group were lower than those in peritoneal experimental group. Group V3 was 20 minutes after administration of the drug (i.e. 20 minutes after modeling). The levels of growth hormone in the control group were lower than those in the P3 group (P0.05) and the growth hormone levels in the V4 group were significantly lower than those in the P4 group (P0.05) 10 minutes, 20 minutes, 30 minutes and 40 minutes after the treatment of the model (P < 0.05), and the level of growth hormone in the V4 group was significantly lower than that in the control group (P0.05). The growth hormone level of the model group was the highest at 30 minutes after modeling. Growth hormone levels in the experimental group (V3-V4 group P3-P5) were the lowest at 20 minutes (30 minutes after modeling). The levels of growth hormone in the V3 group were lower than those in the control group at 20 minutes (that is, 30 minutes after the establishment of the model) and were lower than those in the control group (10 minutes and 30 minutes after the treatment). The levels of growth hormone in P4 group (20 minutes and 40 minutes after model making) (P0.05) were lower than those in P4 group (P 0.05), and the levels of growth hormone in P4 group were lower than those in control group (P 0.05). Conclusion: 1.Methyl ethyl lactol urea can decrease the level of growth hormone in rats in a dose-dependent manner between 0.1mg~10mg. It may be a new type of non-peptide somatostatin. The inhibitory effect of 2.1-methyl-glycosylurea on growth hormone in rats is the best 20 minutes after injection. The inhibitory effect of 3.1-methyl-acetonide on growth hormone in rats The fruit vein approach was superior to the abdominal approach.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 程天明,袁愛力;生長抑素及其類似物的研究進(jìn)展[J];國外醫(yī)學(xué)(腫瘤學(xué)分冊);1998年05期

2 劉春敏,李兆申;生長抑素在消化系統(tǒng)疾病中臨床應(yīng)用進(jìn)展[J];新藥與臨床;1997年03期

3 王松;梁慶模;;非肽類生長抑素類似物研究進(jìn)展[J];中國藥房;2009年19期

本文編號：2090995