本文選題：齊留通 + 花生四烯酸鹽5-脂氧合酶��； 參考：《重慶醫(yī)科大學》2014年碩士論文

【摘要】：目的 探討5-脂氧合酶(5-LO)選擇性抑制劑齊留通(zileuton)對缺血再灌注心肌的保護作用及其可能機制。 方法 健康成年雌性SD大鼠30只，隨機分為假手術組(S組，n=10)、缺血再灌注組(I/R組，n=10)、齊留通組(Z組，n=10)，采用結扎前降支造成缺血45min、松開結扎線灌注120min的方法建立缺血再灌注模型。Z組于手術前15min給予3mg/kg齊留通。再灌注結束后處死動物，HE染色觀察心肌的病理損傷情況，TUNEL法檢測心肌細胞凋亡率，，ELISA法檢測血漿中白三烯B4(LTB4)、IL-1β、TNF-α的含量，Westernblotting和免疫熒光法檢測中性粒細胞中的5-LO分布變化 結果 與I/R組比較，齊留通處理可以明顯減輕缺血再灌注所致的心肌病理損傷，降低心肌細胞凋亡率，降低血漿中炎癥因子LTB4、IL-1β和TNF-α的水平，抑制5-LO的激活轉位，差異有統(tǒng)計學意義(P0.05)，但各組5-LO表達總量無明顯差異(P0.05)。 結論 齊留通對缺血再灌注心肌具有一定的保護作用，其機制可能與抑制5-LO轉位激活，進而減輕炎癥損傷、抑制心肌凋亡有關。
[Abstract]:Objective to investigate the protective effect of 5-lipoxygenase (5-LO) selective inhibitor Zilutong (zileuton) on ischemic reperfusion myocardium and its possible mechanism. Methods 30 healthy adult female SD rats were used. They were randomly divided into three groups: sham operation group (S group), ischemia reperfusion group (I / R group) and Zilutong group (Z group). The model of ischemia reperfusion was established by ligating the descending branch before ligation for 45 minutes and then releasing the ligation line to infuse 120min. The model of ischemia reperfusion was established in group Z before operation. The pathological changes of myocardium were observed by HE staining after reperfusion. Tunel method was used to detect the apoptosis rate of cardiomyocytes. Elisa was used to detect the content of leukotriene B4 (LTB4) IL-1 尾 -TNF- 偽 in plasma. Western blotting and immunofluorescence assay were used to detect the distribution of 5-LO in neutrophils. The results were compared with those in the I / R group. Zilutong treatment could significantly reduce the myocardial pathological injury induced by ischemia and reperfusion, decrease the apoptosis rate of cardiomyocytes, decrease the levels of LTB4- IL-1 尾 and TNF- 偽 in plasma, and inhibit the activation and translocation of 5-LO. The difference was statistically significant (P0.05), but there was no significant difference in the total expression of 5-LO in each group (P0.05). Conclusion Zilutong has a protective effect on myocardial ischemia and reperfusion, and its mechanism may be related to inhibiting the activation of 5-LO translocation, thereby reducing inflammatory injury and inhibiting myocardial apoptosis.
【學位授予單位】：重慶醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R965

【參考文獻】

相關期刊論文 前3條

1 陳占昆;呂厚山;;類風濕關節(jié)炎滑膜細胞中白三烯B4誘導腫瘤壞死因子-α和白細胞介素-1β mRNA表達的測定[J];北京大學學報(醫(yī)學版);2006年05期

2 王陽;米樹華;賈淑杰;周蕓;池U

本文編號：2066323