發(fā)布時間：2018-06-16 18:49

  本文選題：他達拉非 + 移植物閉塞　； 參考：《實用醫(yī)學雜志》2015年11期

【摘要】：目的:探討他達拉非對大鼠慢性移植動脈血管病變保護作用。方法:實驗分3組:同系移植組,供、受者為Lewis大鼠,無處理;異系移植實驗組,Lewis大鼠接受Brown-Norway(BN)大鼠腹主動脈移植;干預組他達拉非0.5 mg/(kg·d),Lewis大鼠接受BN大鼠腹主動脈移植。術(shù)后4周和8周取移植動脈,組織學觀察、測量內(nèi)膜和中膜厚度,Western blot檢測PKG-Ⅰ、Rho A蛋白表達。收集各組血漿,ELISA檢測c GMP濃度。結(jié)果:異系移植實驗組移植動脈呈移植物血管病表現(xiàn),血管內(nèi)膜顯著增厚;干預組血管內(nèi)膜增厚,與實驗組相比顯著減輕(P0.05);干預組血漿c GMP濃度較實驗組顯著增高(P0.05);與實驗組相比,干預組PKG-Ⅰ顯著增高、Rho A明顯降低(P0.05)。結(jié)論他達拉非通過調(diào)控c GMP/PKG-Ⅰ和Rho A通路,抑制移植物血管病發(fā)展。
[Abstract]:Objective: to investigate the protective effect of tadarafil on chronic graft artery disease in rats. Methods: the experiment was divided into three groups: the same transplantation group, the recipient group was Lewis rats without treatment, the experimental group of allogeneic Lewis rats received Brown-NorwayBN rat abdominal aorta transplantation, and the intervention group of tadarafil 0.5 mg/(kg Lewis rats received BN rat abdominal aorta transplantation. The transplanted arteries were harvested at 4 and 8 weeks after operation. The expression of PKG- 鈪，

本文編號：2027750