本文選題：半導體聚合物 + 聚合物納米粒子��； 參考：《湖南師范大學》2014年碩士論文

【摘要】：目前,納米材料已逐漸成為了多門學科研究的基礎。納米材料由于其自身的表面效應、小尺寸效應和宏觀量子隧道效應等,使其在光學、電學、磁學等方面得到了極為廣泛的應用。近來,半導體聚合物納米顆粒(SPNs)因為它們作為熒光探針的突出性質(zhì)如高量子產(chǎn)率、大的光子吸收截面積以及良好的光穩(wěn)定性,已經(jīng)引起了人們越來越大的關注。 本文通過合成不同兩性聚合物配基,并以此作為保護劑,結合納米沉降等方法,探索開發(fā)出水溶性半導體聚合物熒光納米探針。綜合光譜和顯微表征表明,該SPNs具有卓越的光物理學性能,如優(yōu)異的熒光強度,相比量子點減少的光閃爍,比類似大小的熒光蛋白更好的光穩(wěn)定性。更重要的是,由于光學性能的提高,通過把膜生物標記物交聯(lián)到SPNs的表面,發(fā)現(xiàn)它們不僅適用于活細胞標記,而且也適用于單粒子追蹤。具體研究工作主要包括以下幾個方面： 1.優(yōu)化熒光納米顆粒的合成路線,實現(xiàn)小尺寸熒光納米顆粒的合成,首先通過合成不同的兩性聚合物配基,然后以此作為保護劑,結合納米沉降等方法,利用[(9,9-二辛基芴-2,7-二基)-交替-共-(1,4-苯并-{2,1’,3}-噻二唑](PFBT)合成水溶性半導體聚合物熒光納米探針,通過表征發(fā)現(xiàn),合成的納米粒子具有粒徑小、尺寸均勻、熒光強度高等特性。 2.研究合成的半導體聚合物納米顆粒的光學性能,熒光穩(wěn)定性,探討巰基乙胺對其光學性能影響,并在單顆粒層面對其進一步研究。 3.通過所制備的熒光探針顆粒,在活細胞水平上進行腫瘤細胞靶向標記和單顆粒追蹤實驗研究。
[Abstract]:At present, nanomaterials have gradually become the basis of multi-disciplinary research. Nanomaterials have been widely used in optics, electricity and magnetism due to their surface effect, small size effect and macroscopic quantum tunneling effect. Recently, semiconductor polymer nanoparticles (SPNs) have been characterized by their outstanding properties as fluorescent probes such as high quantum yields, large photon absorption cross-sections, and good photostability. In this paper, different amphoteric polymer ligands were synthesized and used as protective agents to explore the development of water-soluble semiconductor polymer fluorescent nanoprobes. The results show that the SPNs have excellent photophysical properties, such as excellent fluorescence intensity, better photostability than the light scintillation reduced by quantum dots, compared with fluorescent proteins of similar size. More importantly, due to the improvement of optical properties, it is found that the membrane biomarkers are not only suitable for living cell labeling, but also suitable for single particle tracing by cross-linking the membrane biomarkers to the surface of SPNs. The specific research work mainly includes the following aspects: 1. The synthesis route of fluorescent nanoparticles was optimized to realize the synthesis of small size fluorescent nanoparticles. Firstly, different amphoteric polymer ligands were synthesized, and then used as protective agents, combined with nano-deposition, etc. The water-soluble semiconductor polymer fluorescence nanoprobes were synthesized by the method of [Dichlorooctylfluorene -2-dioctylfluorene -7- dimethylfluorene -alternating -co-carbolone 4- benzo-4- {2an1zol} -thiadiazole] PFBT.The characterization results show that the synthesized nanoparticles have the characteristics of small size, uniform size and high fluorescence intensity. 2. The results show that the synthesized nanoparticles have the properties of small size, uniform size and high fluorescence intensity. The optical properties and fluorescence stability of the synthesized semiconductor polymer nanoparticles were studied. The effects of mercaptoethylamine on their optical properties were investigated. The target labeling and single particle tracing of tumor cells were studied on the living cell level by using fluorescent probe particles.
【學位授予單位】：湖南師范大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R943;TB383.1

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本文編號：2004371