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  本文選題：多酸 + 吡哌酸�。� 參考：《佳木斯大學(xué)》2014年碩士論文

【摘要】：目的設(shè)計合成出新型的多酸基復(fù)合物，對其進(jìn)行結(jié)構(gòu)表征及生物進(jìn)行研究。探索復(fù)合物的合成規(guī)律，研究其構(gòu)效關(guān)系。 方法復(fù)合物的合成采用水熱合成法；通過X-射線單晶衍射、X-射線粉粉末衍射、元素分析、差熱-熱重和紅外光譜等方法對晶體進(jìn)行結(jié)構(gòu)表征。利用紫外光譜法研究復(fù)合物與DNA的相互作用；采用藥敏紙片法研究復(fù)合物抗菌活性；利用MTT法研究新型復(fù)合物的體外抗腫瘤活性。 結(jié)果合成出4個新的有機活性分子修飾的多酸復(fù)合物；復(fù)合物能夠與DNA發(fā)生相互作用，得到了配合物與DNA的作用模式和相應(yīng)的結(jié)合常數(shù)；復(fù)合物有一定的抗菌活性和抗腫瘤活性。 結(jié)論本論文選擇喹諾酮類藥物分子吡哌酸和唑類雜環(huán)分子作為有機配體，在過渡金屬陽離子存在下，借助配位鍵或分子間相互作用來剪切、修飾或者橋連多酸基本單元，進(jìn)而構(gòu)建新型的多酸基復(fù)合物。研究這類新型復(fù)合物的合成規(guī)律，探索其微觀結(jié)構(gòu)與宏觀性質(zhì)之間的聯(lián)系。制備并表征了4種新型的多酸基復(fù)合物，確定分子式如下： {[Cu（PPA）2]2[H3PMo12O40]}·8H2O （1）； {[Zn（PPA）2][H3PMo12O40]}·[HPPA]·3H2O （2）； {[Zn（HPPA）2H2O]2[H2ZnW12O40]}.9H2O （3）； [Ag3（pytz）2（H2O）]2·[HAgGeMo12O40]·H2O （4）。 復(fù)合物與DNA相互作用的實驗結(jié)果表明，復(fù)合物1-3與DNA具有強的作用力，紫外光譜實驗證明他們與DNA的結(jié)合模式為嵌插結(jié)合。體外抗腫瘤實驗發(fā)現(xiàn)藥物配體自身的性質(zhì)，，多酸自身的構(gòu)型和配位模式，復(fù)合物的結(jié)構(gòu)等許多因素都可能影響復(fù)合物的抗腫瘤活性，即多酸和藥物分子之間的協(xié)同作用調(diào)節(jié)了抗腫瘤活性，其中金屬離子對于電子在多酸與金屬配合物的轉(zhuǎn)移起到了關(guān)鍵作用�？咕鷮嶒灲Y(jié)果表明復(fù)合物1-4對金黃葡萄球菌和大腸桿菌有明顯的抑制作用，其抑菌活性來源與金屬離子Ag和藥物吡哌酸分子。由于復(fù)合物不溶于水可以作為新型固體抗菌材料。
[Abstract]:Objective to design and synthesize a novel polyacid complex and study its structure and biology. To explore the synthesis law of the complex and study its structure-activity relationship. Methods the complex was synthesized by hydrothermal method, and the crystal structure was characterized by X-ray single crystal diffraction powder diffraction, elemental analysis, differential thermogravimetry and infrared spectroscopy. The interaction between the complex and DNA was studied by ultraviolet spectroscopy, the antibacterial activity of the complex was studied by drug sensitive disk method, and the antitumor activity of the new complex was studied by MTT in vitro. Results four new organic active molecular modified polyacid complexes were synthesized, which could interact with DNA, and the interaction mode and binding constants of the complexes with DNA were obtained. The complex has certain antibacterial activity and anti-tumor activity. Conclusion Quinolones were selected as organic ligands. In the presence of transition metal cations, the basic units of polyacids were cut, modified or bridged by coordination bonds or intermolecular interactions. Then a new polyacid complex was constructed. The synthesis law of this kind of new complex is studied, and the relation between its microstructure and macroscopic properties is explored. Four novel polyacid-group complexes were prepared and characterized, and the molecular formula was as follows: {[Cu(PPA)2] 2 [H3PMo12O40]} 8H2O 1; {[Zn(PPA)2] [H3PMo12O40]} [HPPA] 3H2O; {[Zn(HPPA)2H2O] 2 [H2ZnW12O40]}. [Ag3PYTZZZZZZZZZHZ H _ 2O] _ 2 [HAgGeMo12O40] H _ 2O _ (4) H _ 2O _ 4. The experimental results of interaction between complex and DNA show that complex 1-3 has strong interaction with DNA. UV spectra show that their binding mode with DNA is intercalation binding. In vitro antitumor experiments showed that many factors, such as the properties of drug ligands, the configuration and coordination pattern of polyacids, the structure of complexes and so on, may affect the antitumor activity of the complexes. The synergistic action between polyacid and drug molecules regulates the antitumor activity of which metal ions play a key role in the transfer of electrons in polyacid and metal complexes. The results of antibacterial test showed that complex 1-4 had obvious inhibitory effect on Staphylococcus aureus and Escherichia coli, and its antibacterial activity was derived from metal ion Ag and pyrrolidic acid molecule. Because the complex is insoluble in water, it can be used as a new solid antibacterial material.
【學(xué)位授予單位】：佳木斯大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R914.5;R96

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本文編號：1950510