本文選題：慢性腎功能衰竭 + 腎間質(zhì)纖維化　； 參考：《南華大學(xué)》2014年碩士論文

【摘要】：目的觀察白藜蘆醇對單側(cè)輸尿管梗阻大鼠模型腎間質(zhì)纖維化的干預(yù)研究。方法24只清潔級SD大鼠隨機分為假手術(shù)組(A組)和手術(shù)組(B、C),每組各8只,手術(shù)組將大鼠進行單側(cè)輸尿管結(jié)扎的方法建立腎間質(zhì)纖維化的動物模型,再分為模型對照組(B組)、白藜蘆醇治療組(C組)。均予以普通飼養(yǎng),假手術(shù)組A和模型對照組B:以10ml/Kg等量生理鹽水灌胃,白藜蘆醇治療組:給予25mg/kg.d白藜蘆醇(溶解于生理鹽水以10ml/Kg)灌胃,手術(shù)后第14天處死所有大鼠;檢查各組術(shù)后第14天血清尿素氮(BUN)、肌酐(Cr),并觀察各組大鼠UUO術(shù)后14天腎組織纖維化程度:1)用HE和Masson染色的方法觀察腎組織纖維化程度及腎小管上皮細(xì)胞基底膜變化;2)用免疫組織化學(xué)的方法檢測TGF-β1、Col I、Smad2/3、Smad7的蛋白表達水平。結(jié)果1)術(shù)后第14天血清尿素氮、肌酐:模型組較假手術(shù)組大鼠尿素氮、肌酐明顯升高(P0.05),經(jīng)白藜蘆醇治療后,大鼠尿素氮、肌酐明顯下降(P0.05)。2)病理切片HE染色:模型組較假手術(shù)組大鼠腎間質(zhì)損傷指數(shù)明顯升高(P0.01);經(jīng)白藜蘆醇治療后,較模型組其腎間質(zhì)損傷指數(shù)明顯降低(P0.05),較假手術(shù)組腎間質(zhì)損傷指數(shù)增高(P0.01)。3)病理切片Masson染色:模型組膠原含量明顯增高(P0.01),而白藜蘆醇治療后,膠原沉積明顯減輕(P0.01)。4)免疫組化顯示TGF-β1、Col I、Smad2/3、Smad7:假手術(shù)組腎間質(zhì)內(nèi)未見I型膠原表達,模型組可見大量I型膠原沉積,經(jīng)白藜蘆醇治療后,較模型組其腎間質(zhì)內(nèi)I型膠原的表達明顯減輕(P0.05);假手術(shù)組大鼠腎組織TGF-β1呈弱陽性表達,模型組TGF-β1表達較假手術(shù)組顯著增高(P0.01),白藜蘆醇治療組TGF-β1表達較模型組顯著下降(P0.05);假手術(shù)組大鼠腎組織Smad2/3呈弱陽性表達,模型組呈強陽性表達,而白藜蘆醇治療組則表達減弱(P0.01);假手術(shù)組腎組織Smad7表達較模型組明顯增強,而白藜蘆醇治療組則Smad7表達較模型組增強(P0.01)。結(jié)論白藜蘆醇可以通過影響腎組織Col I及TGF-β/Smads通路中Smad2/3、Smad7、TGF-β1表達,抑制腎間質(zhì)纖維化,從而延緩腎功能衰竭。
[Abstract]:Objective to observe the effect of resveratrol on renal interstitial fibrosis in rats with unilateral ureteral obstruction. Methods Twenty-four clean SD rats were randomly divided into sham operation group (n = 8) and operation group (n = 8). Rats in operation group were treated with unilateral ureteral ligation to establish an animal model of renal interstitial fibrosis. They were divided into two groups: model control group B group and resveratrol treatment group C group. All rats were fed with normal feeding, sham operation group (A) and model control group (B) were treated with 10ml/Kg, resveratrol group was given 25mg/kg.d resveratrol (10 ml / kg) dissolved in normal saline. All rats were killed on the 14th day after operation. The serum urea nitrogen bun (bun) and creatinine (Cr) were examined on the 14th day after operation in each group, and the degree of renal fibrosis in each group was observed 14 days after UUO. The degree of renal fibrosis and the basement membrane degeneration of renal tubular epithelial cells were observed by HE and Masson staining. The protein expression of TGF- 尾 1 Col / Smad2 / 3 Smad7 was detected by immunohistochemical method. Results 1) on the 14th day after operation, serum urea nitrogen and creatinine: the urea nitrogen and creatinine in the model group were significantly higher than those in the sham operation group. After resveratrol treatment, the serum urea nitrogen in the model group was significantly higher than that in the sham operation group. He staining of pathological sections: the renal interstitial injury index in the model group was significantly higher than that in the sham operation group, and after resveratrol treatment, the renal interstitial injury index in the model group was significantly higher than that in the sham operation group. Compared with the model group, the renal interstitial injury index of the model group was significantly lower than that of the sham operation group, and the index of renal interstitial injury was higher than that of the sham-operated group. The Masson staining of pathological sections showed that the collagen content in the model group was significantly higher than that in the model group, but after resveratrol treatment, the collagen content in the model group was significantly higher than that in the control group. Collagen deposition significantly alleviated P0.01U. 4) Immunohistochemistry showed that TGF- 尾 _ 1Col / Smad2 / 3 / Smad7: no type I collagen was found in the renal interstitium of sham operation group, and a large number of type I collagen deposition was observed in model group after resveratrol treatment. Compared with the model group, the expression of type I collagen in the renal interstitium was significantly reduced in the model group, and the expression of TGF- 尾 1 was weakly positive in the renal tissue of the sham-operated group. The expression of TGF- 尾 1 in the model group was significantly higher than that in the sham operation group, and the expression of TGF- 尾 1 in the resveratrol treatment group was significantly lower than that in the model group, while the expression of Smad2/3 in the renal tissue of the sham-operated group was weakly positive, and the expression of TGF- 尾 1 in the model group was strongly positive. The expression of Smad7 in renal tissue of sham operation group was significantly higher than that of model group, while the expression of Smad7 in resveratrol treatment group was higher than that in model group. Conclusion resveratrol can inhibit renal interstitial fibrosis and delay renal failure by affecting the expression of Smad2 / 3 Smad7TGF- 尾 1 in Col I and TGF- 尾 / Smads pathway.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R965

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本文編號：1949570