本文選題：非水相 + 蛋白酶�。� 參考：《中國抗生素雜志》2015年06期

【摘要】：目的研究非水相體系中反式頭孢丙烯的酶法合成工藝條件。方法以7-氨基-3-E-丙烯基-3-頭孢菌素-4-羧酸(trans-7-APRA)與對羥基苯甘氨酸(HPG)為底物,利用單甲氧基聚乙二醇修飾化的蛋白酶為催化劑,在非水相體系中合成反式頭孢丙烯。采用單因素實驗確定影響反式頭孢丙烯相對產(chǎn)率的主要因素及其取值范圍。以此為基礎(chǔ),應(yīng)用響應(yīng)面法對底物配比、酶用量、溶劑配比和溫度4因素進行優(yōu)化。結(jié)果非水相體系中反式頭孢丙烯均有一定量的合成,以DMSO和甘油的混合非水相體系為最佳合成體系。蛋白酶非水相合成反式頭孢丙烯的最佳工藝參數(shù):底物配比ntrans-7-APRA:nHPG=7:10,酶用量HPG:Enzyme=1:1,溶劑比率VDMSO:V甘油=2:1,溫度30℃,在此反應(yīng)條件下相對產(chǎn)率可達到63.6%,與理論預(yù)測值相比相對誤差在2%左右。結(jié)論修飾化中性蛋白酶在非水相體系中可有效合成反式頭孢丙烯,該工藝可為酶法制取頭孢丙烯的實際應(yīng)用提供理論依據(jù)。
[Abstract]:Objective to study the conditions of enzymatic synthesis of trans cefpropene in non-aqueous phase system. Methods trans-cefpropene was synthesized from 7-amino-3-E- 3-propenyl -3-cephalosporin-4-carboxylic acid trans-7-APRAand p-hydroxyphenylglycine HPGs using monomethoxypolyethylene glycol modified protease as catalyst in the non-aqueous phase system. Single factor experiment was used to determine the main factors affecting the relative yield of trans cefpropene and its range of values. On the basis of this, the four factors of substrate ratio, enzyme dosage, solvent ratio and temperature were optimized by response surface method. Results there was a certain amount of trans-cefpropene synthesis in the non-aqueous phase system, and the mixture of DMSO and glycerol was the best synthesis system. The optimum technological parameters for the synthesis of trans-cefpropene in the non-aqueous phase of protease are as follows: the ratio of substrates ntrans-7-APRA: nHPGN 7: 10, the amount of enzyme used in the synthesis, the ratio of solvent to glycerol is 2: 1, the temperature is 30 鈩，

本文編號：1934744