本文選題：毛細(xì)管區(qū)帶電泳 + 艾塞那肽�。� 參考：《藥物分析雜志》2015年03期

【摘要】：目的:建立毛細(xì)管區(qū)帶電泳(CZE)方法測(cè)定艾塞那肽純度,并初步考察其穩(wěn)定性。方法:采用未涂層熔融石英毛細(xì)管(48.5 cm×50μm,有效長(zhǎng)度40 cm),考察了運(yùn)行緩沖液p H及濃度、分離電壓、溫度、添加劑乙腈濃度對(duì)分離效果的影響,最終確立電泳條件:運(yùn)行緩沖液為200 mmol·L-1磷酸二氫鈉-磷酸氫二鈉溶液(p H 6.2;含15%乙腈),分離電壓為20 k V,溫度25℃,檢測(cè)波長(zhǎng)214 nm,壓力進(jìn)樣5 k Pa×10 s。結(jié)果:艾塞那肽質(zhì)量濃度在0.05~4.0 mg·m L-1(r=0.999 9)范圍內(nèi)線(xiàn)性關(guān)系良好;檢測(cè)限為3.3μg·m L-1,定量限為8.0μg·m L-1;主峰遷移時(shí)間的日內(nèi)和日間RSD分別為1.07%(n=6)和0.91%(n=9),純度的日內(nèi)和日間RSD分別為0.08%(n=6)和0.10%(n=9)。該方法與HPLC法比較,純度測(cè)定結(jié)果基本一致,本法分離效果更好,分析時(shí)間縮短,能有效檢查艾塞那肽的純度。結(jié)論:該方法可用于艾塞那肽的質(zhì)量控制和穩(wěn)定性研究。
[Abstract]:Objective: to establish a capillary zone electrophoresis (CZE) method for the determination of the purity and stability of Isenapeptide. Methods: the effects of pH and concentration of running buffer, separation voltage, temperature and concentration of additive acetonitrile on the separation were investigated by using uncoated fused quartz capillary tube 48.5 cm 脳 50 渭 m and effective length 40 cm 路m ~ (-1). Finally, the electrophoretic conditions were established: the running buffer solution was 200 mmol L-1 sodium dihydrogen phosphate and sodium hydrogen phosphate solution pH 6.2, the separation voltage was 20 kV, the temperature was 25 鈩，

本文編號(hào)：1928132