本文選題：Halomonas + elongate�。� 參考：《廣東藥學(xué)院學(xué)報(bào)》2016年01期

【摘要】：目的對(duì)具有抗腫瘤活性的海洋細(xì)菌Halomonas elongate進(jìn)行次級(jí)代謝產(chǎn)物的分離、純化及抗腫瘤活性研究。方法通過大孔吸附樹脂、硅膠、ODS、Sephadex LH-20凝膠柱色譜等分離純化手段,對(duì)H.elongate的次級(jí)代謝產(chǎn)物進(jìn)行分離純化;根據(jù)1H NMR譜、13C NMR譜及MS分析,并結(jié)合文獻(xiàn)確定結(jié)構(gòu);采用MTT法對(duì)分離得到的次級(jí)代謝產(chǎn)物進(jìn)行活性檢測(cè)。結(jié)果從海洋細(xì)菌H.elongate的發(fā)酵粗提物中分離鑒定3個(gè)化合物,分別為胸腺嘧啶-2'-脫氧核苷(1)、環(huán)(L-甘氨酸-L-脯氨酸)二肽(2)、1-(2'-脫氧-β-D-赤式-呋喃戊糖)-1氫-1,2,4-三嗪酮(3),活性檢測(cè)結(jié)果顯示3個(gè)化合物對(duì)人肺癌細(xì)胞H1299生長活性均無顯著抑制作用(IC5020μmol/L)。結(jié)論化合物1~3均為首次從Halomonas中分離得到。
[Abstract]:Objective to isolate and purify the secondary metabolites of marine bacteria Halomonas elongate with anti-tumor activity and to study its anti-tumor activity. Methods the secondary metabolites of H.elongate were separated and purified by macroporous adsorption resin, Sephadex LH-20 gel column chromatography, 1H NMR spectrum, 13C NMR and MS analysis. The activity of secondary metabolites was determined by MTT method. Results three compounds were isolated and identified from the fermented crude extracts of marine bacteria H.elongate. They were thymidine (Thymine) -2ON-deoxynucleoside (1), cyclic L- glycine (L-proline) dipeptidyl (Thyopyrimidine), dipeptidyl, deoxiodo- 尾-D- (1-triazinone) -2-triazinone (H1299). The results of activity test showed that the three compounds had no significant inhibitory effect on the growth activity of human lung cancer cell line H1299 by IC5020 渭 mol 路L ~ (-1) 路L ~ (-1). Conclusion compounds 1 and 3 were isolated from Halomonas for the first time.
【作者單位】： 廣東藥學(xué)院中藥學(xué)院;國家海洋局第三海洋研究所海洋生物資源遺傳重點(diǎn)實(shí)驗(yàn)室;
【基金】：國家海洋局第三海洋研究所基本科研業(yè)務(wù)費(fèi)專項(xiàng)資金資助項(xiàng)目(2015028) 海洋經(jīng)濟(jì)創(chuàng)新發(fā)展區(qū)域示范專項(xiàng)經(jīng)費(fèi)資助項(xiàng)目(GD2012-D01-001)
【分類號(hào)】：R915

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