本文選題：大頭金蠅幼蟲 + 抗菌肽　； 參考：《食品科技》2017年10期

【摘要】：目的:從大頭金蠅幼蟲中分離純化天然抗菌肽,并檢測抗菌肽的抑菌活性。方法:提取大頭金蠅幼蟲抗菌肽,粗提物經(jīng)凝膠過濾色譜、陽離子交換樹脂、反相色譜純化,純化產(chǎn)物檢測其抑菌活性并利用GFC-HPLC測定其分子量。結(jié)果:最終純化出一個(gè)活性組分,該組分對(duì)銅綠假單胞菌的最小抑菌濃度為20.40μg/m L,對(duì)金黃色葡萄球菌的最小抑菌濃度為2.04 mg/m L,對(duì)大腸桿菌的最小抑菌濃度為0.17 mg/m L;該活性組分分子量大小為2570 u。結(jié)論:該抗菌肽組分對(duì)銅綠假單胞菌有較強(qiáng)抑菌活性,有較好的開發(fā)利用價(jià)值。
[Abstract]:Objective: to isolate and purify natural antimicrobial peptides from the larvae of Drosophila macrocephala and to detect the antibacterial activity of antimicrobial peptides. Methods: the antimicrobial peptides were extracted from the larvae of Drosophila melanogaster. The crude extracts were purified by gel filtration chromatography, cation exchange resin, reversed phase chromatography. The antibacterial activity of the purified products was determined by GFC-HPLC and its molecular weight was determined by GFC-HPLC. Results: finally, an active component was purified, The minimum inhibitory concentration of this component on Pseudomonas aeruginosa was 20.40 渭 g / mL, on Staphylococcus aureus was 2.04 mg/m / L, on Escherichia coli was 0.17 mg/m / L, and the molecular weight of the active component was 2570 u. Conclusion: the antimicrobial peptide has strong bacteriostatic activity against Pseudomonas aeruginosa and has good value of exploitation and utilization.
【作者單位】： 藥食同源植物資源開發(fā)四川省高校重點(diǎn)實(shí)驗(yàn)室成都大學(xué)四川抗菌素工業(yè)研究所;
【基金】：成都市科技局重大專項(xiàng)(2016-XT00-00023-GX)
【分類號(hào)】：R91
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本文編號(hào)：1894006