本文選題：瑞巴派特 + 阿司匹林　； 參考：《中國病理生理雜志》2017年09期

【摘要】：目的:探討瑞巴派特能否通過促進腸道上皮屏障結(jié)構(gòu)和功能的修復改善阿司匹林導致的小腸黏膜損傷。方法:本研究利用BALB/c小鼠,使用阿司匹林(200 mg·kg~(-1)·d~(-1))連續(xù)5 d灌胃的方法制作小腸損傷模型,并根據(jù)是否誘導小腸損傷及接受瑞巴派特(320 mg·kg~(-1)·d~(-1))的處理將小鼠分為正常對照組、模型組、瑞巴派特對照組及瑞巴派特治療組。通過透射電鏡、免疫組化、qPCR和Western blot等方法,在不同時點系統(tǒng)地觀察瑞巴派特對阿司匹林所致的小鼠小腸黏膜損傷模型中小腸黏膜結(jié)構(gòu)及屏障功能蛋白表達的影響。結(jié)果:阿司匹林所致的小腸黏膜損傷小鼠,經(jīng)瑞巴派特(320 mg·kg~(-1)·d~(-1))連續(xù)5 d處理后,小腸上皮細胞間緊密連接結(jié)構(gòu)的損害明顯減輕,小腸組織中緊密連接蛋白ZO-1及occludin在mRNA及蛋白質(zhì)水平的表達均明顯增加(P0.05),環(huán)氧化酶2(COX-2)和增殖相關信號分子β-catenin、c-Myc的表達也高于對照組(P0.05),組織勻漿中前列腺素E_2濃度顯著增加(P0.05),而COX-1的表達在治療組中無明顯改變。同時,治療組血清中的D-乳酸水平明顯降低(P0.05)。結(jié)論:瑞巴派特能夠通過上調(diào)COX-2的表達,促進阿司匹林所致的小鼠小腸黏膜損傷的修復,改善腸屏障的結(jié)構(gòu)與功能。
[Abstract]:Aim: to investigate whether repapet can improve the intestinal mucosal injury induced by aspirin by promoting the repair of intestinal epithelial barrier structure and function. Methods: in this study, BALB/c mice were perfused with aspirin (200 mg / kg) for 5 days to make the model of small intestine injury. The mice were divided into normal control group and model group according to whether to induce small intestine injury and to be treated with repapet (320 mg / kg) DX. Repapet control group and repapet treatment group. By means of transmission electron microscope (TEM), immunohistochemical staining (Western blot) and QPCR, the effects of repapet on the expression of mucosal structure and barrier protein in small and medium-sized intestine of aspirin induced intestinal mucosal injury in mice were systematically observed at different time points. Results: the damage of tight junction between intestinal epithelial cells in aspirin induced intestinal mucosal injury in mice treated with ribavirin 320 mg / kg / d for 5 days was significantly alleviated. The expression of ZO-1 and occludin in small intestine increased significantly in mRNA and protein levels, and the expression of cyclooxygenase 2 (COX-2) and proliferation-related signal molecule 尾 -cateninine c-Myc was also higher than that of control group (P0.05), and the concentration of prostaglandin E _ 2 in tissue homogenate was significantly higher than that in control group (P0.05), and the expression of 尾 -cateninine c-Myc in tissue homogenate was significantly higher than that in control group. The expression of COX-1 did not change in the treatment group. At the same time, the serum D-lactate level in the treatment group was significantly lower than that in the control group (P 0. 05). Conclusion: repapet can improve the structure and function of intestinal barrier by upregulating the expression of COX-2 and promoting the repair of small intestinal mucosal injury induced by aspirin in mice.
【作者單位】： 贛州市人民醫(yī)院消化內(nèi)科;中山大學孫逸仙紀念醫(yī)院消化內(nèi)科;
【基金】：國家自然科學基金資助項目(No.81370475)
【分類號】：R965

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