本文選題：赭曲霉 + 洋地黃毒苷　； 參考：《天然產(chǎn)物研究與開發(fā)》2015年03期

【摘要】：以赭曲霉Rn405轉(zhuǎn)化洋地黃毒苷的微生物轉(zhuǎn)化工藝為研究對象,對助溶劑種類、底物濃度、金屬離子種類和濃度等關(guān)鍵參數(shù)進(jìn)行優(yōu)化,確定的最優(yōu)轉(zhuǎn)化條件為:發(fā)酵培養(yǎng)基中添加1 mmol/L Mn2+離子,28℃,180 rpm振蕩培養(yǎng)28 h時(shí)加入溶解于DMSO的底物溶液,使其終濃度為100 mg/L,然后在相同的條件下轉(zhuǎn)化96 h。此時(shí),底物的轉(zhuǎn)化率和11α-羥基洋地黃毒苷元的生成率分別達(dá)到了100%和25.4%,比優(yōu)化前分別提高了33.1%和14.5%。通過斯氏灌流法和Langendorff灌流法,探討兩個(gè)轉(zhuǎn)化產(chǎn)物對蟾蜍和大鼠心肌細(xì)胞收縮能力的影響。結(jié)果表明,洋地黃毒苷元對心肌細(xì)胞收縮率的影響和底物洋地黃毒苷相當(dāng),而11α-羥基洋地黃毒苷元對心肌細(xì)胞收縮率的影響比底物洋地黃毒苷和洋地黃毒苷元均有所增強(qiáng)。
[Abstract]:The microbial transformation of digitoxin from Aspergillus ochratus Rn405 was studied. The key parameters, such as the type of solvent, the concentration of substrate, the type and concentration of metal ions, were optimized. The optimal transformation conditions were determined as follows: adding 1 mmol/L Mn2 ion at 28 鈩，

本文編號：1846970