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  本文選題：超聲波輔助酸解 + 淀粉納米顆粒�。� 參考：《甘肅農(nóng)業(yè)大學》2017年碩士論文

【摘要】：本論文以馬鈴薯淀粉為原料,用超聲波輔助酸解法制備淀粉納米顆粒,研究了超聲功率、硫酸濃度和酸解時間對顆粒粒徑及產(chǎn)率的影響,得出最佳制備工藝參數(shù),并對制備的淀粉納米顆粒的理化性質(zhì)進行表征;以制備的淀粉納米顆粒為載體材料,濕法制粒得到5-氨基水楊酸的藥物片芯,研究了片芯在模擬人工胃腸道環(huán)境中的緩釋效果;對緩釋片芯經(jīng)過腸溶包衣后,研究了包衣片在模擬胃腸道環(huán)境中藥物的累積釋放百分率,并對其進行體外釋藥動力學模型擬合;以大腸桿菌為指示菌,通過測量抑菌圈的大小,驗證了不同時間點5-氨基水楊酸緩釋包衣片的藥物釋放程度,研究結(jié)果如下:1.用超聲波對原淀粉進行預(yù)處理后再進行酸水解,分析所得產(chǎn)物的粒徑、產(chǎn)率得出最佳制備工藝參數(shù)為:在40℃條件下,用3 mol/L硫酸水解15%的淀粉乳,攪拌速度為100 r/min,超聲功率400 W,酸解20 h后,得到的淀粉納米顆粒平均粒徑在50~80 nm范圍內(nèi),產(chǎn)率為14.1%。2.將原淀粉與最佳工藝參數(shù)制備的淀粉納米顆粒相比,結(jié)晶度由21.57%增長到46.35%,吸水率由34.8%增長到96.9%,吸附率由49.1%增長到93.7%,比表面積由0.1789 m2/g增長到1.6491 m2/g,氮氣吸附量由0.68374 cm3/g增長到1.34734 cm3/g,說明淀粉納米顆粒具有強大的表面能和吸附性能。3.以5-ASA為主藥,分別用原淀粉和淀粉納米顆粒為載體材料,濕法制粒得到片芯,在模擬人工胃腸液中用透析法進行釋放試驗,統(tǒng)計不同釋放介質(zhì)中的累積釋藥百分率。結(jié)果表明:以淀粉納米顆粒為載體材料片芯的累計釋藥百分率均低于以原淀粉為載體材料的片芯,因此,納米淀粉具有明顯的緩釋效果。4.對緩釋片芯進行腸溶包衣后得到緩釋包衣片,將其置于模擬人工胃腸環(huán)境中,統(tǒng)計不同釋放介質(zhì)中藥物的累積釋藥百分率,對藥物釋放率進行模型擬合。結(jié)果表明:緩釋包衣片在模擬人工胃腸環(huán)境中的累積釋藥百分率與釋藥時間的擬合模型呈對數(shù)形式的緩慢釋放,符合緩釋片劑非恒速釋放的一級釋藥動力學模型。5.以大腸菌為指示菌,用抑菌試驗去驗證緩釋包衣片體外釋放試驗后藥物的釋放程度。結(jié)果表明:緩釋包衣片在模擬胃腸環(huán)境中的累積釋藥率持續(xù)增大,在反應(yīng)15 h后累積釋藥率達到87.1%,抑菌圈為25.67 mm,說明藥物在結(jié)腸液中大量釋放,發(fā)揮了藥物在結(jié)腸的藥效。
[Abstract]:This paper uses potato starch as raw material to prepare starch nanoparticles by ultrasonic assisted acid hydrolysis. The effects of ultrasonic power, sulfuric acid concentration and acid hydrolysis time on particle size and yield are studied. The optimum processing parameters are obtained. The physicochemical properties of the prepared starch nanoparticles are characterized. The prepared starch nanoparticles are prepared. The drug core of 5- amino salicylic acid was obtained by wet granulation. The release effect of the core in the simulated artificial gastrointestinal environment was studied. The cumulative release percentage of the drugs in the simulated gastrointestinal tract was studied after the release of the release core in the intestinal tract. By measuring the size of bacteriostasis, the degree of drug release of 5- amino salicylic acid sustained-release coated tablets at different time points was verified. The results were as follows: 1. the original starch was pretreated with ultrasonic wave after acid hydrolysis, and the particle size of the product was analyzed. The best preparation parameters were obtained at 40 degrees centigrade. 3 mol/L sulfuric acid hydrolyzed 15% starch milk, stirring speed was 100 r/min, ultrasonic power was 400 W, and acid solution 20 h, the average particle size of starch nanoparticles was within 50~80 nm range. The yield was 14.1%.2., the crystallinity increased from 21.57% to 46.35%, and the water absorption rate increased from 34.8% to 96., compared with the starch nanoparticles prepared by the optimum process parameters. 9%, the adsorption rate increased from 49.1% to 93.7%, the specific surface area increased from 0.1789 m2/g to 1.6491 m2/g, and the nitrogen adsorption capacity increased from 0.68374 cm3/g to 1.34734 cm3/g, indicating that the starch nanoparticles have strong surface energy and adsorption property.3. with 5-ASA as the main drug, respectively, using the raw starch and amylum nanoparticles as the carrier material, and the wet granulation to get the chip core. In the simulated artificial gastrointestinal fluid, the release test was carried out by dialysis, and the cumulative release percentage in different release media was calculated. The results showed that the cumulative release percentage of the core with starch nanoparticles as the carrier was lower than that of the raw starch as the carrier material. Therefore, the nanoscale starch had a significant release effect of.4. to the sustained release tablets. The core was coated with enteric coated coated tablets and placed in the simulated artificial gastrointestinal environment. The cumulative release percentage of drugs in different release media was calculated and the drug release rate was fitted. The results showed that the fitting model of the cumulative release rate of the sustained release coating tablets in the simulated artificial gastrointestinal environment and the time of drug release was on the basis of the fitting model. The slow release of several forms was in line with the first order release kinetics model of the sustained release tablets,.5., with Escherichia coli as the indicator. The release degree of the drug released after the release test in vitro was verified by the bacteriostasis test. The results showed that the cumulative release rate of the sustained-release coated tablets in the simulated gastrointestinal environment increased continuously, after the reaction of 15 h. The cumulative release rate reached 87.1% and the inhibition zone was 25.67 mm, indicating that the drug was released in colon juice and played a role in the colon.

【學位授予單位】：甘肅農(nóng)業(yè)大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R943

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