本文選題：妥布霉素 + 地塞米松　； 參考：《中國藥房》2015年33期

【摘要】：目的:建立同時測定妥布霉素地塞米松滴眼液中妥布霉素和地塞米松含量的方法。方法:采用高效液相色譜-柱前衍生化-雙波長法。色譜柱為Agilent C18,流動相為0.25%三羥甲基氨基甲烷-乙腈-0.5 mol/L硫酸(40∶59∶1,V/V/V),流速為1.0 ml/min,檢測波長為365、220 nm,柱溫為30℃,進(jìn)樣量為20μl。結(jié)果:妥布霉素、地塞米松檢測質(zhì)量濃度線性范圍分別為18.01~180.09、6.02~60.24μg/ml(r=0.999 5、0.999 8);精密度、穩(wěn)定性、重復(fù)性試驗(yàn)的RSD1%;加樣回收率分別為98.65%~100.01%、98.92%~100.20%,RSD分別為0.47%、0.50%(n=9)。結(jié)論:該方法簡便省時、專屬性強(qiáng)、重復(fù)性好、結(jié)果準(zhǔn)確可靠,可用于妥布霉素地塞米松滴眼液的質(zhì)量控制。
[Abstract]:Objective: to establish a method for simultaneous determination of tobramycin and dexamethasone in tobramycin dexamethasone eye drops. Methods: high performance liquid chromatography-pre-column derivatization-double wave length method was used. The chromatographic column was Agilent C18, the mobile phase was 0.25% trihydroxymethyl aminomethane -acetonitrile 0.5 mol/L sulfuric acid 40: 591V / V / V / V, the flow rate was 1.0ml / min, the detection wavelength was 365220 nm, the column temperature was 30 鈩，

本文編號：1828470