發(fā)布時(shí)間：2018-04-28 01:26

  本文選題：納米二氧化鈦 + 胃癌　； 參考：《中國(guó)生物醫(yī)學(xué)工程學(xué)報(bào)》2017年03期

【摘要】：利用Cole-Cole模型評(píng)價(jià)不同劑量納米二氧化鈦(Nano-TiO_2)的細(xì)胞毒性作用,并探討電生理機(jī)制。150和300 mg/L的Nano-TiO_2懸液作用人胃癌MGC803細(xì)胞24 h后,制備成細(xì)胞懸浮液。在1 k Hz~100 MHz范圍,采用Agilent 4294A精密阻抗分析儀測(cè)量了人胃癌MGC803細(xì)胞懸浮液電阻抗的幅值和相位角,經(jīng)電阻抗頻譜和Nyquist圖的曲線擬合的殘差分析,建立Cole-Cole模型參數(shù),評(píng)估Nano-TiO_2對(duì)MGC803細(xì)胞導(dǎo)電性能的影響。結(jié)果表明,150和300 mg/L的Nano-TiO_2引起第一電阻抗增量(ΔZ1)分別減少18.18%(P0.001)和39.39%(P0.001),第二電阻抗增量(ΔZ2)分別減少6.56%(P0.001)、8.2%(P0.001),降低了MGC803細(xì)胞膜及其核膜的電阻,增加了其導(dǎo)電性能;第一特征頻率(fC1)分別增加19.74%(P0.001)和29.67%(P0.001),第二特征頻率(fC2)分別增加6.28%(P0.001)、23.43%(P0.001);第一散射角(β1)分別降低1.35%(P0.05)和2.70%(P0.05)。ColeCole模型可評(píng)價(jià)Nano-TiO_2的細(xì)胞毒性作用并解釋其電生理機(jī)制,為納米顆粒的細(xì)胞毒理研究提供一種電特性方法。
[Abstract]:Cole-Cole model was used to evaluate the cytotoxicity of different doses of Nano-TiO-2 (Nano-TiO-2), and to investigate the electrophysiological mechanism. 150 mg/L and 300 mg/L Nano-TiO_2 suspension were used to treat MGC803 cells for 24 h, and then the suspension of human gastric cancer MGC803 cells was prepared. In the range of 1 k Hz~100 MHz, the amplitude and phase angle of suspension impedance of human gastric cancer MGC803 cells were measured by Agilent 4294A precise impedance analyzer. The parameters of Cole-Cole model were established by residual analysis of impedance spectrum and Nyquist curve fitting. To evaluate the effect of Nano-TiO_2 on the electrical conductivity of MGC803 cells. The results showed that the first electrical impedance increment (螖 Z _ 1) and the second impedance increment (螖 Z _ 2) were reduced by 18.18 and 39.39, respectively, and the second impedance increment (螖 Z _ 2) decreased by 6.56 ~ (-1) P _ (0.001) (P _ (0.001) (P _ (0.001), respectively, which decreased the resistance of MGC803 cell membrane and its nuclear membrane, and increased its electrical conductivity. The first characteristic frequency (fC1) and the second characteristic frequency (FC2) increased by 19.74g / P0.001) and 29.67kb / P0.001a, respectively, and the second characteristic frequency (fC2) increased by 6.28 and 23.43p respectively, and the first scattering angle (尾 _ 1) decreased by 1.35g / P0.05) and the 2.70%(P0.05).ColeCole model could be used to evaluate the cytotoxicity of Nano-TiO_2 and explain its electrophysiological mechanism. To provide a method for the study of cellular toxicology of nanoparticles.
【作者單位】： 寧波大學(xué)醫(yī)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(51277099) 寧波市科技惠民項(xiàng)目(2015C5009)
【分類號(hào)】：R99

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