發(fā)布時間：2018-04-27 21:45

  本文選題：藥用輔料 + 橄欖油�。� 參考：《藥物分析雜志》2015年11期

【摘要】：目的:分析油脂類輔料橄欖油中甾醇組成及質(zhì)量分?jǐn)?shù)。方法:首先從橄欖油中提取不皂化物,運(yùn)用高效液相色譜法從不皂化物中分離并收集甾醇的餾分。液相色譜條件:采用Kromasil 60-5 SIL硅膠柱(250 mm×4.6 mm,5μm),以異丙醇-正己烷(1∶99,v/v)為流動相等度洗脫,流速1.0 m L·min-1,檢測波長210 nm,進(jìn)樣量20μL。然后對餾分進(jìn)行衍生化,采用氣相色譜法檢測各甾醇的組成及百分含量;氣相色譜條件:采用DB-5毛細(xì)管柱(30 m×0.25 mm×0.25μm),進(jìn)樣口溫度:290℃;進(jìn)樣量:2~3μL;分流比為25∶1;載氣:氦氣;流速1.8 m L·min-1;程序升溫:起始柱溫260℃,保持50 min,再以5℃·min-1的速率升溫至290℃,保持5 min。結(jié)果:橄欖油中含有多種甾醇,如膽甾醇、菜子甾醇;菜油甾醇、豆甾醇、△7-菜油甾醇、△5,23-豆甾二烯醇、赤桐甾醇、β-谷甾醇、谷甾烷醇、△5-燕麥甾醇、△5,24-豆甾二烯醇、△7-豆甾醇等,其中β-谷甾醇的含量比較高,占總甾醇量的80%以上,其次是菜油甾醇,△5,24-豆甾二烯醇等。結(jié)論:本法可以對油脂類輔料橄欖油中的甾醇譜進(jìn)行分析,為油脂類藥用輔料植物油中甾醇譜的研究提供了參考。
[Abstract]:Objective: to analyze the composition and mass fraction of sterol in olive oil. Methods: the unsaponifiable compounds were extracted from olive oil and sterol fractions were separated and collected by high performance liquid chromatography (HPLC). The liquid chromatographic conditions were as follows: Kromasil 60-5 SIL silica gel column (250mm 脳 4.6mm), isopropanol-n-hexane (1: 9: 9 v / v) as the flow equality elution, flow rate 1.0 mL min-1, detection wavelength 210nm, sample volume 20 渭 L. The composition and percentage content of sterols were determined by gas chromatography. The gas chromatographic conditions were as follows: DB-5 capillary column: 30m 脳 0.25 mm 脳 0.25 渭 m, sample temperature: 290 鈩，

本文編號：1812464