本文選題：超高效液相色譜法 + 纈沙坦氫氯噻嗪片��； 參考：《中國藥房》2017年15期

【摘要】：目的:建立同時測定纈沙坦氫氯噻嗪片中纈沙坦和氫氯噻嗪含量的方法。方法:采用超高效液相色譜法。色譜柱為Phenomenex C_(18),流動相為[0.1%磷酸溶液-乙腈(95∶5,V/V)]-[0.1%磷酸溶液-乙腈(5∶95,V/V)](梯度洗脫),流速為0.25 mL/min,檢測波長為272 nm,柱溫為35℃,進樣量為1.5μL。結(jié)果:纈沙坦和氫氯噻嗪檢測質(zhì)量濃度線性范圍分別為8.1~324.2μg/mL(r=0.999 9)、1.2~50.1μg/mL(r=0.999 9);定量限分別為0.24、0.04 ng,檢測限分別為0.06、0.01 ng;精密度、穩(wěn)定性、重復(fù)性試驗的RSD2.0%;加樣回收率分別為97.69%~100.35%(RSD=1.03%,n=9)、98.27%~100.60%(RSD=0.83%,n=9)。結(jié)論:該方法操作簡單、快速,結(jié)果準確,可用于纈沙坦氫氯噻嗪片中纈沙坦和氫氯噻嗪含量的同時測定。
[Abstract]:Objective: to establish a method for simultaneous determination of valsartan and hydrochlorothiazide in valsartan hydrochlorothiazide tablets. Methods: ultrahigh performance liquid chromatography was used. The chromatographic column was Phenomenex Che 18, the mobile phase was [0.1% phosphoric acid solution-acetonitrile 95: 5 V / V]-[0.1% phosphoric acid solution-acetonitrile 5: 95 V / V] (gradient elution, flow rate was 0.25 mL / min, detection wavelength was 272nm, column temperature was 35 鈩，

本文編號：1811654