本文選題：三苯雙脒 + 代謝物�。� 參考：《中國(guó)藥房》2015年02期

【摘要】：目的:建立同時(shí)測(cè)定人尿液中三苯雙脒代謝物氨脒和乙酰氨脒濃度的方法,并進(jìn)行其排泄動(dòng)力學(xué)研究。方法:8名健康男性受試者空腹口服三苯雙脒腸溶片400 mg,收集服藥前及服藥后0~2、2~4、4~6、6~8、8~12、12~24、24~36、36~48 h的尿液。尿樣經(jīng)沉淀蛋白并稀釋后,以比卡魯胺為內(nèi)標(biāo),采用高效液相色譜法進(jìn)樣測(cè)定,色譜柱為Agilent Zorbax Extend C18(250 mm×4.6 mm,5μm)流動(dòng)相為乙腈-水-三乙胺(60∶40∶0.2,V/V/V),紫外檢測(cè)波長(zhǎng)為265 nm,流速為0.7 ml/min,進(jìn)樣量為10μl。結(jié)果:氨脒和乙酰氨脒尿藥濃度分別在0.5~500μg/ml(r=0.996 2)和0.1~200μg/ml(r=0.995 8)范圍內(nèi)線性關(guān)系良好,最低定量限分別為0.5、0.1μg/ml,絕對(duì)回收率分別為94.78%~95.23%和91.26%~96.99%,相對(duì)回收率為98.37%~101.44%和99.26%~102.06%,日內(nèi)、日間RSD7%;-20℃冷凍24 h及7 d和反復(fù)凍融均穩(wěn)定,RSD7%。受試者口服400 mg三苯雙脒腸溶片后,氨脒和乙酰氨脒48 h尿累積排泄率分別為(55.84±13.60)%和(14.98±7.65)%,采用DAS 2.0程序計(jì)算氨脒和乙酰氨脒半衰期分別為(3.46±0.73)h和(4.21±0.43)h,尿藥排泄速率常數(shù)分別為(0.21±0.07)h-1和(0.17±0.02)h-1。結(jié)論:該方法簡(jiǎn)單、快速、靈敏、重復(fù)性好,可用于健康受試者口服三苯雙脒腸溶片后氨脒和乙酰氨脒尿藥濃度的測(cè)定。
[Abstract]:Objective: to establish a method for simultaneous determination of three benzamid metabolites of amamamid and Acetamiprid in human urine and to study its excretory kinetics. Methods: 8 healthy male subjects were taken orally Tribendimidine Enteric-coated Tablets 400 mg orally, and the urine of 0~2,2~4,4~6,6~8,8~ 12,12~24,24~36,36~48 h before and after taking the medicine was collected. The urine sample was precipitated protein. After dilution, using high performance liquid chromatography with biacaramine as internal standard, the chromatographic column was Agilent Zorbax Extend C18 (250 mm * 4.6 mm, 5 u m) with acetonitrile water three ethylamine (60: 40: 0.2, V/V/V), UV detection wavelength 265 nm, velocity of 0.7 mL /min, and 10 mu L. results: amamamid and acetamiprid urine concentration. The linear relationship between 0.5~500 mu g/ml (r=0.996 2) and 0.1~200 mu g/ml (r=0.995 8) is good, the minimum quantitative limit is 0.5,0.1 u g/ml, and the absolute recovery is 94.78%~95.23% and 91.26%~96.99% respectively. The relative recovery rate is 98.37%~101.44% and 99.26%~102.06%, day, day, 24, 7 and repeated freezing and thawing. After oral administration of 400 mg Tribendimidine Enteric-coated Tablets, the cumulative excretion rate of amamamid and acetamiprid 48 h urine was (55.84 + 13.60)% and (14.98 + 7.65)% respectively. The half-life of amamamid and acetamiprid was (3.46 + 0.73) H and (4.21 + 0.43) h respectively by DAS 2 program. The urinary excretion rate constant was (0.21 + 0.07) H-1 and (0.17 + 7.65) h-1. conclusion, respectively. The method is simple, rapid, sensitive and reproducible, and can be used for the determination of amamiprid and Acetamiprid in healthy volunteers after oral administration of Tribendimidine Enteric-coated Tablets.

【作者單位】： 山東醫(yī)學(xué)高等�？茖W(xué)校;山東大學(xué)齊魯醫(yī)院臨床藥理研究所;德州市食品藥品監(jiān)督管理局德城區(qū)分局;
【分類號(hào)】：R927;O657.72

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 肖樹(shù)華,吳惠敏,王，

本文編號(hào)：1810333