本文選題：加壓毛細管電色譜 + 定量分析�。� 參考：《中國抗生素雜志》2015年05期

【摘要】：目的采用反相加壓毛細管電色譜法定量測定依維莫司含量。方法使用反相色譜柱,以p H5.5的2mmol/L磷酸緩沖液(Na2HPO4-Na H2PO4)-乙腈(30∶70,V/V)為流動相,檢測波長為278nm、分離電壓為-16k V、流速為0.08m L/min,柱溫49℃,等度洗脫。結果經方法學考察確定最佳條件,在該條件下,依維莫司濃度在0.3~5.0mg/m L范圍內線性良好(R2=0.9992,n=6);檢出限(S/N=3)和定量限(S/N=10)分別為3.9和7.8μg/m L;日間和日內精密度均小于5%;加樣回收率均高于98%。結論該方法簡單方便、準確、靈敏、可靠,適用于依維莫司的定量分析。
[Abstract]:Objective to establish a reversed-phase pressure capillary electrochromatography method for quantitative determination of ivermostr. Methods A reversed-phase column was used. The mobile phase was Na2HPO4-NaH2PO4-NaH2PO4-acetonitrile 30: 70V / V). The detection wavelength was 278nm, the separation voltage was -16kV, the flow rate was 0.08mL / min, the column temperature was 49 鈩，

本文編號：1799431