發(fā)布時(shí)間：2018-04-24 13:43

  本文選題：多肽微陣列 + 金納米粒子探針�。� 參考：《分析化學(xué)》2015年02期

【摘要】：研制了一種基于多肽微陣列芯片的熒光和共振光散射雙通路檢測方法,對(duì)血液樣品中的凝血酶抑制劑進(jìn)行檢測。以生物素化的多肽微陣列芯片為反應(yīng)平臺(tái),加入凝血酶水解多肽上的特異位點(diǎn)使其C末端的生物素解離,通過親和素和生物素之間的特異性結(jié)合,用熒光探針和30 nm金納米粒子探針標(biāo)記此反應(yīng)過程。凝血酶抑制劑阻止凝血酶對(duì)底物多肽的水解反應(yīng),通過熒光和共振光散射信號(hào)強(qiáng)度的變化檢測抑制劑的抑制能力。酶溶液和加標(biāo)人血清中測定的半抑制濃度(IC50)值:阿加曲班抗凝血酶Ⅲ4-(2-氨乙基)苯磺酰氟鹽酸鹽,IC50差的絕對(duì)值隨抑制劑特異性的減弱而增大。當(dāng)抑制劑濃度為7.5μmol/L時(shí),血漿中5種化合物的抑制能力:阿加曲班抗凝血酶Ⅲ胰蛋白酶抑制劑N-(反式-環(huán)氧丁二�；�)-L-亮氨酸-4-胍基丁基酰胺4-(2-氨乙基)苯磺酰氟鹽酸鹽。在血漿中考察了阿加曲班和抗凝血酶Ⅲ的可逆性。對(duì)比熒光法和共振光散射法在血液樣品中的檢測結(jié)果,用30 nm金納米粒子標(biāo)記的共振光散射法更適用于復(fù)雜血液樣品中抑制劑的檢測
[Abstract]:A dual fluorescence and resonance light scattering (RLS) method based on polypeptide microarray was developed to detect thrombin inhibitors in blood samples. Using biotin microarray microarray as the reaction platform, the specific site on the thrombin hydrolyzed polypeptide was added to dissociate the biotin from the C-terminal of the peptide, and the specific binding between avidin and biotin was obtained. Fluorescence probe and 30 nm gold nanoparticles probe were used to label the reaction process. Thrombin inhibitor blocked the hydrolysis of the substrate polypeptide by thrombin and detected the inhibition ability of the inhibitor by the changes of fluorescence and resonance light scattering (RLS) signal intensity. The absolute value of IC50 difference of agatoban antithrombin 鈪，

本文編號(hào)：1796883