本文選題：固相萃取 + 高效液相色譜法�。� 參考：《中國藥房》2015年34期

【摘要】：目的:測定大鼠體內(nèi)咖啡因、氨苯砜和氯唑沙宗的血藥濃度,計(jì)算藥動學(xué)參數(shù)。方法:取大鼠6只,ig咖啡因、氨苯砜和氯唑沙宗混合溶液,給藥劑量分別為1.5、2、3 mg/kg,于給藥前及給藥后0.5、1、2、3、4、6、8、12、24 h取血0.2~0.3 ml;血漿樣品用固相萃取法處理,采用高效液相色譜法,以N-(2-羥乙基)鄰苯二甲酰亞胺為內(nèi)標(biāo),測定咖啡因、氨苯砜和氯唑沙宗的血藥濃度;并用DAS 2.0軟件計(jì)算藥動學(xué)參數(shù)。結(jié)果:咖啡因、氨苯砜及氯唑沙宗檢測質(zhì)量濃度的線性范圍均為0.2~30μg/ml(r分別為0.996 4、0.996 1、0.998 8),定量限均為0.2μg/ml,低、中、高質(zhì)量濃度水平的方法回收率分別為(84.8±3.6)%~(111.4±10.2)%(RSD為4.3%~9.8%)、(107.0±13.3)%~(113.5±8.1)%(RSD為7.1%~14.0%)、(104.2±10.8)%~(111.1±12.2)%(RSD為8.0%~11.0%)(n=3);藥動學(xué)參數(shù)tmax為(1.70±0.99)、(1.50±1.00)、(1.92±0.80)h,t1/2為(0.73±0.22)、(2.77±1.35)、(2.78±2.34)h,cmax為(2.60±0.50)、(5.78±1.19)、(9.76±1.37)mg/L,AUC0-t為(8.43±0.79)、(20.68±1.91)、(26.71±2.45)mg·h/L(n=6)。結(jié)論:本法操作簡便、靈敏度高、結(jié)果準(zhǔn)確,可用于咖啡因、氨苯砜和氯唑沙宗的血藥濃度測定和藥動學(xué)研究。
[Abstract]:Aim: to determine the plasma concentrations of caffeine, dapsone and chlorzoxazone in rats and calculate the pharmacokinetic parameters. Methods: a mixture of caffeine, dapsone and chlorzoxazone was taken from 6 rats. The dosage was 1.5 mg / kg. The blood samples were collected before and 24 hours after administration. The plasma samples were treated by solid phase extraction and HPLC, respectively. The plasma concentrations of caffeine, dapsone and chlorzoxazone were determined with N-chloride-2-hydroxyethyl phthalimide as internal standard, and the pharmacokinetic parameters were calculated by DAS 2.0 software. Results: the linear range of caffeine, dapsone and chlorzoxazone was 0.996 渭 g/ml(r and 0.998 8 渭 g/ml(r, respectively. The limit of quantification was 0.2 渭 g / ml. 楂樿川閲忔祿搴︽按騫崇殑鏂規(guī)硶鍥炴敹鐜囧垎鍒負(fù)(84.8鹵3.6)%~(111.4鹵10.2)%(RSD涓，

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