本文選題：二甲雙胍 + 低氧損傷��； 參考：《軍事醫(yī)學(xué)》2017年08期

【摘要】：目的探討二甲雙胍對低氧誘導(dǎo)的大鼠腎上腺嗜鉻細(xì)胞瘤PC12細(xì)胞損傷的影響及其可能的作用機制。方法利用低氧手套箱(0.3%O2)處理PC12細(xì)胞,并采用甲紫染色和Annexin-Ⅴ/碘化丙啶(PI)雙標(biāo)法檢測細(xì)胞生存及凋亡情況;采用CCK8法測定細(xì)胞活力;蛋白印跡檢測信號通路活化水平及蛋白表達(dá)水平。結(jié)果低氧(0.3%O2)可對PC12細(xì)胞產(chǎn)生時間依賴性的損傷效應(yīng);二甲雙胍預(yù)處理可對低氧造成的PC12細(xì)胞損傷產(chǎn)生保護(hù)效應(yīng),在0.3~1 mmol/L劑量范圍內(nèi),其保護(hù)作用具有劑量依賴性;二甲雙胍能激活細(xì)胞內(nèi)腺苷酸活化蛋白激酶(AMP-activated protein kinase,AMPK)信號通路;AMPK激酶選擇性抑制劑復(fù)合物C預(yù)處理細(xì)胞后,阻斷了二甲雙胍對PC12細(xì)胞的低氧損傷的保護(hù)作用。結(jié)論二甲雙胍預(yù)處理對低氧造成的PC12細(xì)胞損傷具有保護(hù)作用,AMPK激酶在二甲雙胍保護(hù)低氧造成的PC12細(xì)胞損傷中起著重要作用。
[Abstract]:Objective to investigate the effect of metformin on PC12 cell damage induced by hypoxia in rat adrenal pheochromocytoma and its possible mechanism. Methods PC12 cells were treated with hypoxia glove box (0.3g / O2). The survival and apoptosis of PC12 cells were detected by using the method of nail violet staining and Annexin- 鈪，

本文編號：1787982