本文選題：納米銀 + 半通道　； 參考：《北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版)》2017年03期

【摘要】：目的:探討納米銀(silver nanoparticles,AgNPs)對(duì)皮膚細(xì)胞半通道活性的影響,并進(jìn)一步研究半通道活性的改變?cè)贏gNPs誘導(dǎo)的細(xì)胞增殖抑制中的作用。方法:不同濃度(0、0.1、0.2、1.0、5.0、10.0、15.0和20.0μg/cm~2)的AgNPs處理人永生化表皮細(xì)胞系(HaCaT)24 h,采用碘化丙啶(propidium iodide,PI)染料排除法測(cè)定細(xì)胞活性,采用溴化乙錠(ethidium bromide,EB)攝取法測(cè)定細(xì)胞半通道活性。通過以常規(guī)密度(4×10~4個(gè)/cm~2)或低密度(1×10~4個(gè)/cm~2)接種細(xì)胞及采用半通道抑制劑甘珀酸(carbenoxolone,CBX)預(yù)處理的方法調(diào)節(jié)細(xì)胞半通道活性,采用細(xì)胞增殖-毒性檢測(cè)試劑盒(cell counting kit-8,CCK-8)檢測(cè)半通道活性受抑制、不同濃度AgNPs作用后的HaCaT細(xì)胞的增殖水平。結(jié)果:當(dāng)AgNPs濃度低于10μg/cm~2時(shí),HaCaT細(xì)胞的細(xì)胞活性未受明顯影響。10μg/cm~2的AgNPs作用2、12和24 h可導(dǎo)致細(xì)胞半通道活性升高至對(duì)照組的116.67%、124.85%和139.53%,與對(duì)照組相比差異均具有統(tǒng)計(jì)學(xué)意義(P0.01),且25、50和100μmol/L CBX預(yù)處理可明顯抑制10μg/cm~2AgNPs作用24 h后的細(xì)胞的半通道活性(P0.01)。此外,100μmol/L CBX預(yù)處理可升高低密度和常規(guī)密度下10μg/cm~2AgNPs作用24 h后細(xì)胞的增殖水平,其中,低密度下,CBX預(yù)處理組與AgNPs單獨(dú)處理組相比,細(xì)胞增殖水平顯著升高(P0.01)。結(jié)論:一定濃度的AgNPs可激活HaCaT細(xì)胞的半通道,半通道活性升高在AgNPs引發(fā)的細(xì)胞增殖抑制效應(yīng)中具有重要作用。
[Abstract]:Aim: to investigate the effects of silver silver nanoparticlesclines (Ag NPs) on the half-channel activity of skin cells, and to investigate the role of the changes of half-channel activity in the inhibition of cell proliferation induced by AgNPs. Methods: human immortalized epidermal cell line HaCaT was treated with AgNPs at different concentrations of 0.1g / cm ~ (2) and 20.0 渭 g / cm ~ (-2) respectively. The cell activity was determined by propidium iodide PII dye exclusion method, and the half-channel activity was measured by ethidium bromidee uptake method. Cells were inoculated with conventional density of 4 脳 10 ~ 4 / cm ~ (-2) or low density of 1 脳 10 ~ (4) / cm ~ (-2) and treated with carbenoxolone (CBX), a half-channel inhibitor, to regulate the cell half-channel activity. Cell counting kit-8 CCK-8 was used to detect the inhibition of half-channel activity and the proliferation level of HaCaT cells treated with different concentrations of AgNPs. Results: when the concentration of AgNPs was lower than 10 渭 g / cm ~ 2, the cell activity of HaCaT cells was not significantly affected by AgNPs of 10 渭 g/cm~2 for 2 h and 24 h, and the half-channel activity increased to 116.67 ~ 124.85% and 139.53% of the control group. The difference was statistically significant compared with the control group. Pretreatment with 25 渭 mol/L and 100 渭 mol/L CBX significantly inhibited the half-channel activity of P0.01G after 24 h treatment with 10 渭 g/cm~2AgNPs. In addition, preconditioning with 100 渭 mol/L CBX could increase the proliferation level of cells treated with 10 渭 g/cm~2AgNPs at low density and normal density for 24 h, and the proliferation level of cells in low density pretreated group was significantly higher than that in AgNPs alone group. Conclusion: a certain concentration of AgNPs can activate the half-channel of HaCaT cells, and the increase of half-channel activity plays an important role in the inhibition of cell proliferation induced by AgNPs.
【作者單位】： 北京大學(xué)公共衛(wèi)生學(xué)院勞動(dòng)衛(wèi)生與環(huán)境衛(wèi)生學(xué)系;
【基金】：國家自然科學(xué)基金(21077006,21477004) 國家高技術(shù)研究發(fā)展計(jì)劃(863計(jì)劃,2012AA062804)資助~~
【分類號(hào)】：R99

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