本文選題：吩噻嗪類光敏劑YWW + 病原體滅活�。� 參考：《中國輸血雜志》2015年06期

【摘要】：目的探討酚噻嗪類光敏劑YWW007應(yīng)用于血液病原體滅活的安全使用劑量。方法細(xì)胞毒性試驗(yàn):使用YWW007終濃度為1、4、12、20、40μmol/L培養(yǎng)基培養(yǎng)小鼠成纖維細(xì)胞,用四甲基偶氮唑鹽比色(MTT)方法檢測細(xì)胞存活率;遺傳毒性評價(jià):采用YWW007終濃度為1、2、4、6、8、12μmol/L的培養(yǎng)基培養(yǎng)小鼠淋巴瘤細(xì)胞,用微孔板法做tk基因突變試驗(yàn),計(jì)算平板效率、相對存活率以及TFT抗性突變頻率等指標(biāo)。結(jié)果當(dāng)YWW007濃度達(dá)到40μmol/L時(shí),具有明顯的細(xì)胞毒性;而YWW007濃度為12、4、2和1μmol/L時(shí),細(xì)胞存活率70%,提示細(xì)胞毒性輕微;YWW007濃度≤4μmol/L時(shí),在含有和不含有代謝活化系統(tǒng)(S9)條件下,其突變頻率(MF)值均未達(dá)到陰性對照組MF值的2倍,未見劑量-效應(yīng)關(guān)系。結(jié)論 YWW007濃度≤4μmol/L為用于病原體滅活的安全劑量。
[Abstract]:Objective to investigate the safe dosage of phenothiazide Guang Min agent YWW007 in inactivation of blood pathogens.Methods Cytotoxicity test: mouse fibroblasts were cultured on the YWW007 medium with the final concentration of 1g 412C 2040 渭 mol/L. The survival rate of the cells was determined by tetramethyl azolium colorimetric assay (MTT).Genotoxicity evaluation: murine lymphoma cells were cultured on a YWW007 medium of 12 渭 mol/L. The tk gene mutation test was performed by microplate method. The plate efficiency, relative survival rate and TFT resistance mutation frequency were calculated.Results when the concentration of YWW007 reached 40 渭 mol/L, the cytotoxicity was obvious, but when the concentration of YWW007 was 12 渭 mol/L and 1 渭 mol/L, the cell survival rate was 70%, suggesting that when the concentration of YWW007 was less than 4 渭 mol/L, when the concentration of YWW007 was less than 4 渭 mol/L, when the concentration of YWW007 was less than 4 渭 mol/L, the cell survival rate was 70% when the concentration of YWW007 was 12 渭 mol/L and 1 渭 mol/L, respectively.The mutation frequency and MF value were not twice as high as those of the negative control group, and there was no dose-effect relationship.Conclusion YWW007 concentration 鈮，

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