本文選題：聚乙烯亞胺 + 胎牛血清��； 參考：《中國藥房》2015年16期

【摘要】：目的:研究胎牛血清(FBS)對聚乙烯亞胺(PEI)基因傳遞系統(tǒng)的降毒作用。運(yùn)用Nanoparticles albumin bound技術(shù)的原理,制備以FBS修飾PEI/DNA的復(fù)合物(PEI/DNA/FBS)。采用MTT比色法計(jì)算人宮頸癌上皮Hela細(xì)胞存活率(CV),測定PEI質(zhì)量濃度分別為0.5、1、2、5、10、15、20、30μg/ml時(shí)的細(xì)胞毒性,比較FBS含量分別為0、2.5%、5%、10%時(shí)PEI/FBS復(fù)合物和N(PEI中氮的物質(zhì)的量)/P(DNA中磷的物質(zhì)的量)比分別為10、20時(shí)的PEI/DNA/FBS復(fù)合物的細(xì)胞毒性差異,比較PEI和N/P比分別為10、20且FBS含量為0、5%、10%的PEI/DNA/FBS復(fù)合物作用4、8、12、16 h時(shí)的細(xì)胞毒性差異。結(jié)果:CV值與PEI質(zhì)量濃度呈負(fù)相關(guān),當(dāng)PEI≥10μg/ml時(shí),對細(xì)胞有明顯的毒性;隨FBS含量的增加CV值增加,當(dāng)FBS含量為2.5%、5%、10%時(shí)PEI/DNA/FBS復(fù)合物CV值均在80%以上,且FBS含量為5%、10%時(shí)CV值均大于100%;且隨作用時(shí)間的延長CV值基本維持穩(wěn)定。結(jié)論:FBS可有效降低PEI基因傳遞系統(tǒng)的細(xì)胞毒性,經(jīng)FBS修飾后的PEI基因傳遞系統(tǒng)不會(huì)隨作用時(shí)間的延長而加大對細(xì)胞的損傷。
[Abstract]:Aim: to study the antiviral effect of fetal bovine serum FBS on the transmission system of polyethylene imine (PEI) gene.Using the principle of Nanoparticles albumin bound technique, the FBS modified PEI/DNA complex PEI / DNA / FBSs was prepared.The survival rate of human cervical cancer epithelial Hela cells was calculated by MTT colorimetric method. The cytotoxicity of PEI was measured when the mass concentration of PEI was 0.5 ~ (-1) ~ 10 ~ (10) C ~ (15) ~ (20) ~ (30) 渭 g/ml, respectively.To compare the cytotoxicity of the PEI/FBS complex and the nitrogen substance in N(PEI, the ratio of PEI/FBS complex to the amount of phosphorus in N(PEI at 10 and 20:00, respectively, was compared with that of the PEI/DNA/FBS complex with the content of 0, 2.5 and 5, respectively, and the difference of cytotoxicity of the PEI/DNA/FBS complex at 10 and 20:00, respectively.To compare the cytotoxicity of PEI/DNA/FBS complex with PEI and N / P ratio of 10 ~ 20 and FBS content of 0 ~ 5 ~ 10% at 16 h.Results there was a negative correlation between the CV value of PEI and the mass concentration of PEI. When PEI 鈮，

本文編號(hào)：1753324