本文選題：亞肉座菌 + 代謝物��； 參考：《菌物學(xué)報(bào)》2017年05期

【摘要】：亞肉座菌能侵染粉虱和介殼蟲(chóng),具有悠久的生物防治歷史,但關(guān)于其天然產(chǎn)物的種類(lèi)及抗腫瘤活性研究尚不深入。本研究借助硅膠柱、HPLC等色譜技術(shù)從亞肉座菌發(fā)酵液中分離純化到6個(gè)化合物�；诤舜殴舱窈唾|(zhì)譜數(shù)據(jù)解析,化合物1 6依次鑒定為:5α,8α-表二氧-24(R)-甲基膽甾-6,22-二烯-3β-醇5α,8α-epidioxy-24(R)-methylcholesta-6,22-diene-3β-ol(1),杜斯塔寧dustanin(2),3β-乙酸基-15α,22-二羥基何伯烷3β-acetoxy-15α,22-dihydroxyhopane(3),麥角固醇ergosterol(4),7β,15α,22-三羥基何伯烷7β,15α,22-trihydroxyhopane(5)和5-(羥甲基)呋喃-3-羧酸flufuran(6),化合物1和6為首次從蟲(chóng)生真菌中獲得。對(duì)化合物進(jìn)行抗腫瘤活性測(cè)試發(fā)現(xiàn),化合物1和5對(duì)人肝癌細(xì)胞(BEL-7404)展示顯著的抗腫瘤活性,IC50值分別為28μmol/L和12.4μmol/L,而它們對(duì)人正常肝臟細(xì)胞(HL-7702)和腎臟上皮細(xì)胞(HEK-293T)均未呈現(xiàn)出明顯活性。該研究對(duì)闡明亞肉座菌代謝產(chǎn)物及其活性提供了重要數(shù)據(jù),為開(kāi)發(fā)抗腫瘤新藥提供了理論參考。
[Abstract]:It has a long history of biological control, but the research on the species and antitumor activity of its natural products has not been further studied.In this study, six compounds were isolated and purified from fermentation broth by silica gel column HPLC.Based on NMR and MS data,鍖栧悎鐗，

本文編號(hào)：1752054