本文選題：缺血再灌注 + 右美托咪定�。� 參考：《中國臨床藥理學雜志》2017年20期

【摘要】：目的研究右美托咪定(Dex)預處理對缺血再灌注大鼠心肌細胞凋亡及凋亡蛋白表達的影響。方法按照體重將雄性SD大鼠隨機分為4組,每組7只:低高2個劑量實驗組(結扎前0.5 h分別腹腔注射Dex 10,30μg·kg~(-1)),模型組及假手術組(結扎前0.5 h均腹腔注射0.9%Na Cl 5 m L·kg~(-1))。用結扎左冠狀動脈前支構建心肌缺血-再灌注損傷大鼠模型。以缺口末端標記(TUNEL)法檢測各組大鼠心肌細胞凋亡率;以免疫組化法檢測各組大鼠心肌組織中Bcl-2及Bax蛋白表達情況。結果假手術組、模型組與低高2個劑量實驗組的心肌細胞凋亡率(AR,%)分別為0.42±0.06,19.73±4.12,13.31±3.65,10.04±2.71;這4組的心肌組織Bcl-2陽性表達指數(shù)(PEI)分別為0.25±0.03,5.74±1.06,11.44±2.01,14.71±2.13;這4組的心肌組織Bax PEI分別為0.27±0.04,19.95±5.01,15.02±4.82,12.13±3.14;這4組的Bcl-2/Bax分別為0.93±0.05,0.29±0.04,0.76±0.06,1.20±0.07。與假手術組比較,模型組大鼠心肌細胞AR、Bcl-2、Bax PEI及Bcl-2/Bax的差異均有統(tǒng)計學意義(均P0.01);與模型組比較,低高2個劑量實驗組大鼠心肌組織這4項指標差異均有統(tǒng)計學意義(均P0.01)。結論 Dex可通過上調(diào)Bcl-2蛋白表達及下調(diào)Bax蛋白表達,抑制心肌缺血-再灌注損傷大鼠心肌細胞的凋亡。
[Abstract]:Aim to study the effects of dexmetomidine Dexpreconditioning on myocardial apoptosis and apoptotic protein expression in rats with ischemia reperfusion.Methods male Sprague-Dawley rats were randomly divided into 4 groups according to their body weight. The rats in each group were divided into four groups: the low and high dose groups (Dex 1030 渭 g / kg) were injected intraperitoneally at 0.5 h before ligation, and the model group and sham-operation group (0.5 h before ligation) were injected intraperitoneally with 0.9%Na Cl 5 m L 路kg ~ (-1).The rat model of myocardial ischemia reperfusion injury was established by ligating the anterior branch of left coronary artery.The apoptosis rate of cardiac myocytes in each group was detected by Tunel Nick end labeling and the expression of Bcl-2 and Bax protein in myocardium of each group was detected by immunohistochemical method.緇撴灉鍋囨墜鏈粍,妯″瀷緇勪笌浣庨珮2涓墏閲忓疄楠岀粍鐨勫績鑲岀粏鑳?yōu)鍑嬩骸鐜?AR,%)鍒嗗埆涓，

本文編號：1737989